DEVELOPMENT AND COMMISSIONING OF MICROBEAM CAPABILITIES ON BL12-2

BL12-2 上微束功能的开发和调试

• 批准号: 8362091
• 负责人: S M SOLTIS
• 金额: $ 19.14万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362091
• 关键词: Address; Algorithms; Collimator; Development; Feedback; Funding; Grant; Helium; Housing; National Center for Research Resources; Noise; Principal Investigator; Procedures; Radiation; Research; Research Infrastructure; Resources; Sampling; Software Tools; Source; System; Temperature; Testing; United States National Institutes of Health; base; cost; instrument; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Microbeam mode for studying microcrystals is being developed and implemented for BL12-2. A precision microdiffractometer, developed in-house, is used as the base instrument. An in-line camera is incorporated into the beam line for viewing microcrystals along the beam axis, which reduces misalignments due to parallax. The beam line setup also includes an automated collimation system. Software tools were developed for the microbeam collimation system and the in-line camera. An automated alignment procedure of the microbeam (15 um x 5 um) to the phi axis was implemented. Controls for selecting the microbeam mode and viewing and centering samples with the in-line video camera were implemented in Blu-Ice. The diffraction based crystal centering routine was modified for centering microcrystals. An algorithm to accurately distinguish between diffraction and noise is currently being tested. A significant effort continues to be aimed at addressing beam stability challenges, such as refinement of a helium path system, temperature control system, x-ray beam attenuator and mirror pitch feedback system.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 用于研究微晶体的微束模式正在为 BL12-2 开发和实施。 使用内部开发的精密微衍射仪作为基础仪器。 光束线中集成了一个在线相机，用于沿光束轴观察微晶体，从而减少因视差引起的错位。 The beam line setup also includes an automated collimation system.为微束准直系统和在线相机开发了软件工具。 实施了微束（15 um x 5 um）与 phi 轴的自动对准程序。 在 Blu-Ice 中实现了选择微束模式以及使用在线摄像机查看和居中样品的控制。 基于衍射的晶体定心程序被修改以用于定心微晶体。 目前正在测试一种准确区分衍射和噪声的算法。 我们继续付出巨大努力来解决光束稳定性挑战，例如氦路径系统、温度控制系统、X 射线光束衰减器和镜面间距反馈系统的改进。