IN-SITU UV/VIS SPECTROSCOPY AND CRYSTALLOGRAPHY

原位紫外/可见光谱和晶体学

• 批准号: 8362094
• 负责人: S M SOLTIS
• 金额: $ 8.22万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362094
• 关键词: Crystallography; Data; Development; Funding; Grant; In Situ; Measurement; National Center for Research Resources; Principal Investigator; Radiation; Research; Research Infrastructure; Resources; Robotics; Source; Spectrum Analysis; System; Temperature; United States National Institutes of Health; X ray diffraction analysis; X-Ray Diffraction; absorption; base; cost; cytochrome ba3; high throughput screening; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Measurement of the auto-reduction rate by the x-ray beam was continued for the oxidized form of cytochrome ba3. It involves combined X-ray diffraction data and single crystal absorption spectra in the UV/Visible region were recorded at different temperatures. Based on these measurements the rate of reduction is determined. The project drives development of compatibility between the UV/VIS spectroscopy system and the robotic mounting system for high-throughput screening.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 对于细胞色素ba3的氧化形式，继续通过X射线束测量自还原率。 它涉及组合的 X 射线衍射数据和在不同温度下记录的紫外/可见光区域的单晶吸收光谱。 根据这些测量结果确定减少率。 该项目推动了紫外/可见光谱系统和用于高通量筛选的机器人安装系统之间兼容性的开发。