AMILORIDE ATTENUATES APOPTOSIS INDUCED BY DRUGS & GAMMA RADIATION

阿米洛利是一种K-节约型利尿剂，可有效抑制 大多数哺乳动物细胞质膜上的Na/H转运体。我们 研究了阿米洛利对细胞凋亡进程的影响 喜树碱(CAM)、放线菌酮(CHX)和联合化疗药物对HL-60细胞的诱导作用 20Gy伽玛射线照射。所提供的数据表明， 阿米洛利对细胞膜Na/H转运体活性及细胞内pH的影响 可能会潜在地影响凋亡信号级联，导致 延缓细胞凋亡性死亡的进展速度。 正在进行的研究也指出了细胞内pH的参与 和钙在调节细胞凋亡核酸内切酶活性中的作用，以及 需要一个功能性的Na/H交换器来诱导细胞凋亡。

Amiloride is a K+-sparing diuretic that effectively inhibits the Na+/H+ transporter in the plasma membrane of most mammalian cells. We have examined the effects of amiloride on the progression of apoptosis in HL-60 cells induced by camptothecin (CAM), cycloheximide (CHX) and 20 Gy gamma irradiation. Data presented show that effects of amiloride on membrane Na+/H+ transporter activity and intracellular pH can potentially affect apoptotic signaling cascades, leading to a retardation in the rate of progression to an apoptotic cell death. Studies in progress also point to the involvement of intracellular pH and Ca2+ in the regulation of apoptotic endonuclease activity, and the need for a functional Na+/H+ exchanger for the induction of apoptosis.