AMILORIDE ATTENUATES APOPTOSIS INDUCED BY DRUGS & GAMMA RADIATION

阿米洛利减弱药物引起的细胞凋亡

• 批准号: 6280915
• 负责人: JOSE COBO
• 金额: $ 0.71万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6280915
• 关键词: biomedical resource; human tissue; nucleic acids; proteins

Amiloride is a K+-sparing diuretic that effectively inhibits the Na+/H+ transporter in the plasma membrane of most mammalian cells. We have examined the effects of amiloride on the progression of apoptosis in HL-60 cells induced by camptothecin (CAM), cycloheximide (CHX) and 20 Gy gamma irradiation. Data presented show that effects of amiloride on membrane Na+/H+ transporter activity and intracellular pH can potentially affect apoptotic signaling cascades, leading to a retardation in the rate of progression to an apoptotic cell death. Results also point to the involvement of intracellular pH and Ca2+ in the regulation of apoptotic endonuclease activity, and the need for a functional Na+/H+ exchanger for the induction of apoptosis

阿米洛利是一种保留 K+ 的利尿剂，可有效抑制 大多数哺乳动物细胞质膜中的 Na+/H+ 转运蛋白。 我们 研究了阿米洛利对细胞凋亡进展的影响 在喜树碱 (CAM)、放线菌酮 (CHX) 和 20 Gy 伽马射线照射。 提供的数据表明，效果 阿米洛利对膜 Na+/H+ 转运蛋白活性和细胞内 pH 的影响 可能会影响细胞凋亡信号级联，导致 细胞凋亡进展速度减慢。 结果还表明细胞内 pH 值和 Ca2+ 参与 凋亡核酸内切酶活性的调节，以及对细胞凋亡的需要 诱导细胞凋亡的功能性 Na+/H+ 交换器