REDUCTION OF RADIATION INDUCED CELL CYCLE ARREST BY AMILORIDE

用流式细胞仪检测阿米洛利对细胞周期的影响 对细胞周期进程的影响，以及阿米洛利对 减弱2.5、5.0诱导的S和G2期检查点反应 和7.5Gy伽马辐射。已故的S相位延迟和 辐射诱导的G2期阻滞均显著减少 阿米洛利处理的样本没有明显的细胞凋亡或坏死 24小时为一周期。结果表明，阿米洛利可降低 辐射诱导HL-60细胞G2期阻滞几乎等量 以及咖啡因，而且在更大程度上比星形孢子素。 阿米洛利对细胞内离子浓度的影响 可能导致钙依赖的信号级联反应的改变，从而 减少辐射介导的细胞周期扰动。

Flow cytometry were used to investigate the effects of amiloride on cell cycle progression, and the effectiveness of amiloride to attenuate the S and G2 phase checkpoint responses induced by 2.5, 5.0 and 7.5 Gy of gamma radiation. The late S phase delay and the radiation-induced G2 block were both significantly reduced in amiloride-treated samples without apparent apoptosis or necrosis over a 24 h period. Results indicate that amiloride reduces the radiation-induced G2 block in HL-60 cell populations almost equally well as caffeine, and to a greater extent than staurosporine. Alterations in intracellular ion concentrations induced by amiloride may lead to changes in Ca2+-dependent signaling cascades, and thereby decrease the radiation-mediated cell cycle perturbations.