AMILORIDE ATTENUATES APOPTOSIS INDUCED BY DRUGS & GAMMA RADIATION

阿米洛利是一种保钾利尿剂，可有效抑制 大多数哺乳动物细胞质膜上的Na+/H+转运蛋白。 我们 研究了阿米洛利对细胞凋亡进程的影响 在喜树碱（CAM）、环己酰亚胺（CHX）和 20戈伊伽马辐照。 数据显示， 阿米洛利对细胞膜Na+/H+转运体活性和细胞内pH的影响 可能会影响凋亡信号级联，导致 进展至凋亡性细胞死亡的速率的延迟。 正在进行的研究还指出，细胞内pH值的参与， 和Ca ~（2+）在调节凋亡核酸内切酶活性中的作用， 需要功能性Na+/H+交换器来诱导细胞凋亡。

Amiloride is a K+-sparing diuretic that effectively inhibits the Na+/H+ transporter in the plasma membrane of most mammalian cells. We have examined the effects of amiloride on the progression of apoptosis in HL-60 cells induced by camptothecin (CAM), cycloheximide (CHX) and 20 Gy gamma irradiation. Data presented show that effects of amiloride on membrane Na+/H+ transporter activity and intracellular pH can potentially affect apoptotic signaling cascades, leading to a retardation in the rate of progression to an apoptotic cell death. Studies in progress also point to the involvement of intracellular pH and Ca2+ in the regulation of apoptotic endonuclease activity, and the need for a functional Na+/H+ exchanger for the induction of apoptosis.