REDUCTION OF RADIATION INDUCED CELL CYCLE ARREST BY AMILORIDE

阿米洛利减少辐射诱导的细胞周期阻滞

• 批准号: 6327962
• 负责人: JOSE COBO
• 金额: $ 0.31万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6327962
• 关键词: biological products; biomedical resource; technology /technique

Flow cytometry was used to investigate the effects of amiloride on cell cycle progression, and the effectiveness of amiloride to attenuate the S and G2 phase checkpoint responses induced by 2.5, 5.0 and 7.5 Gy of gamma radiation. The late S phase delay and the radiation-induced G2 block were both significantly reduced in amiloride-treated samples without apparent apoptosis or necrosis over a 24 h period. Results indicate that amiloride reduces the radiation-induced G2 block in HL-60 cell populations almost equally well as caffeine, and to a greater extent than staurosporine. Alterations in intracellular ion concentrations induced by amiloride may lead to changes in Ca2+-dependent signaling cascades, and thereby decrease the radiation-mediated cell cycle perturbations. Future studies are designed to determine the effects of amiloride on the expression of various cyclins, particularly cyclins A and B1.

采用流式细胞术检测阿米洛利对体外培养的人肝癌细胞系的影响。 细胞周期进程，以及阿米洛利对 减弱由2.5、5.0 和7.5戈伊的伽马射线 后期S相延迟和 辐射诱导的G2阻滞均显著减少， 阿米洛利处理的样品没有明显的凋亡或坏死， 24小时周期。 结果表明，阿米洛利减少了 HL-60细胞群中辐射诱导的G2阻滞几乎同样 以及咖啡因，并且在更大程度上比星形孢菌素。 阿米洛利引起的细胞内离子浓度的变化 可能导致Ca 2+依赖性信号级联的变化，从而 减少辐射介导的细胞周期扰动。 未来 设计研究以确定阿米洛利对 各种细胞周期蛋白，特别是细胞周期蛋白A和B1的表达。