MARINER INSTRUMENT TESTING & MODIFICATION

质谱仪可以采用几种不同方法中的一种， 质量分析，包括飞行时间（TOF）。 迄今为止，大多数TOF 分析器已经采用来自电离的离子的线性注入 进入质量分析仪。 这种模式的缺点 操作包括增加动能分布， 将电离过程转化为加速电离所产生的能量 潜力 此外，亚稳或碰撞的中性产物 分解可能对背景信号有贡献。 新 仪器（水手）正在开发的珀金埃尔默生物系统 来规避这些问题。 这些采用电喷雾电离 在四极透镜中进行碰撞冷却， 加速进入TOF。 我们与珀金埃尔默合作， 优化使用这些仪器进行各种分析， 问题，使用直接注入样品和接口， 毛细管柱HPLC TOF分析仪提供高分辨率数据 和精确的质量测量;我们正在开发算法， 优化这些信息在肽和蛋白质分析中的应用。 （在协作项下报告的额外工作和仪器时间） 项目和其他技术研究和开发项目。

Mass spectrometers can employ one of several different methods for mass analysis, including time of flight (TOF). To date, most TOF analyzers have employed linear injection of ions from the ionization region into the mass analyzer. Disadvantages of this mode of operation include the addition of the kinetic energy distribution from the ionization process onto the energy imparted by the accelerating potential. Also, the neutral products from metastable or collisional decomposition can contribute to the background signal. New instruments (Mariner) are being developed by Perkin Elmer Biosystems to circumvent these problems. These employ electrospray ionization with collisional colling in a quadrupole lens followed by orthogonal acceleration into the TOF. We are working with Perkin Elmer to optimize the use of these instruments for a variety of analytical problems, using both direct infusion of samples and interfacing to capillary column HPLC. The TOF analyzer gives high resolution data and accurate mass measurements; we are developing algorithms to optimize the use of this information for peptide and protein analysis. (Additional effort and instrument time reported under Collaborative projects and other Technical Research and Development projects.)