MARINER INSTRUMENT TESTING & MODIFICATION

海员仪器测试

• 批准号: 6120202
• 负责人: ALMA L BURLINGAME
• 金额: $ 1.22万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120202
• 关键词: biological products; biomedical equipment development; biomedical resource; biotechnology; drug adverse effect; human tissue; spectrometry; structural biology; technology /technique

Mass spectrometers can employ one of several different methods for mass analysis, including time of flight (TOF). To date, most TOF analyzers have employed linear injection of ions from the ionization region into the mass analyzer. Disadvantages of this mode of operation include the addition of the kinetic energy distribution from the ionization process onto the energy imparted by the accelerating potential. Also, the neutral products from metastable or collisional decomposition can contribute to the background signal. New instruments (Mariner) are being developed by Perkin Elmer Biosystems to circumvent these problems. These employ electrospray ionization with collisional colling in a quadrupole lens followed by orthogonal acceleration into the TOF. We are working with Perkin Elmer to optimize the use of these instruments for a variety of analytical problems, using both direct infusion of samples and interfacing to capillary column HPLC. The TOF analyzer gives high resolution data and accurate mass measurements; we are developing algorithms to optimize the use of this information for peptide and protein analysis. (Additional effort and instrument time reported under Collaborative projects and other Technical Research and Development projects.)

质谱计可以使用几种不同的方法之一来 质量分析，包括飞行时间(TOF)。到目前为止，大多数TOF 分析员使用了电离离子的线性注入 区域添加到质谱仪中。这种模式的缺点是 操作包括将动能分布从 加速产生的能量上的电离过程 潜力。此外，亚稳或碰撞产生的中性产物 分解可以对背景信号做出贡献。新的 Perkin Elmer生物系统公司正在开发仪器(Mariner) 来绕过这些问题。它们采用电喷雾电离。 四极透镜中的碰撞冷却和正交化 加速进入TOF。我们正在与Perkin Elmer合作 优化这些仪器的使用，以进行各种分析 问题，既使用直接样本输入，也使用接口连接 毛细管柱高效液相色谱法。TOF分析仪提供高分辨率数据 和精确的质量测量；我们正在开发算法来 优化这些信息在多肽和蛋白质分析中的使用。 (在Collaborative项下报告的额外工作量和仪器时间 项目和其他技术研究与开发项目。)