SEQUENCING MHC CLASS I PEPTIDES FROM TRYPANOSOMA CRUZI: CHAGAS DISEASE

我们正在测序主要组织相容性复合体（MHC）类 来自克氏锥虫感染细胞的I肽。 T.克鲁济河 一种原生动物寄生虫携带的reduviid错误，是一个原因， 南美洲的慢性心脏病，称为南美锥虫病 疾病 不幸的是，这种疾病不能治疗，除非 使用有害的化疗。 当人类细胞遇到蛋白质时， 例如入侵寄生虫分泌的外源蛋白， 在细胞表面呈现蛋白水解肽片段 与MHC蛋白相关。 通过纯化这些肽， 免疫亲和层析，一组目前可用的HPLC 技术，以及气相分离技术，我们可以在 我们希望能够通过质谱分析 感染特异性MHC I类肽， T细胞介导的免疫学可以用来对抗这种致命的疾病。 疾病

We are sequencing the major histocompatability complex (MHC) class I peptides from Trypanosoma cruzi-infected cells. T. cruzi, a protozoan parasite carried by the reduviid bug, is the cause of a debilitating chronic heart disease in South America known as Chagas disease. Unfortunately, this disease cannot be treated except by using harmful chemotherapy. When a human cell encounters a protein, such as the foreign protein secreted by an invading parasite, it presents proteolytic peptide fragments on the surface of the cell associated with the MHC protein. By purifying these peptides using immuno-affinity chromatography, a battery of currently available HPLC techniques, and the gas-phase separation techniques available to us in mass spectrometry, we hope to be able to identify and sequence infection-specific MHC class I peptides so that a vaccine harnessing T-cell-mediated immunology can be developed to fight this fatal disease.