Labelled IMS TAG Proteins for Quantitative Mass Spectrometry Imaging

该项目旨在开发生物组织中蛋白质定量质谱成像（MSI）的方法。将使用两种非动物模型，这两种模型都包括由人类细胞系制成的多细胞三维（3D）组织构建体;我们实验室开发的新型肿瘤球体模型和商业3D人类皮肤构建体。该方法的成功开发将为使用动物模型代表人类肿瘤和皮肤的体内和体外测试提供替代方案。所开发的方法也将适用于动物组织，其中与采用免疫组织化学和免疫荧光的常规技术相比，成像和定量多种非标记蛋白质的能力有可能减少许多程序中使用的动物数量。

This project aims to develop methodology for the quantitative Mass Spectrometry Imaging (MSI) of proteins in biological tissue. Two non-animal models will be used both of which comprise multi-cellular, three-dimensional (3D) tissue constructs fabricated from human cell lines; a novel type of tumour spheroid model developed in our laboratory and a commercial 3D human skin construct Successful development of the methodology will present an alternative to both in vivo and ex vivo testing using animal models to represent human tumours and skin. The developed methodology will also be applicable to animal tissue where the ability to image and quantify multiple non-labelled proteins has the potential to reduce the number of animals used in many procedures, in comparison to conventional techniques employing immunohistochemistry, and immunoflouresence.