Labelled IMS TAG Proteins for Quantitative Mass Spectrometry Imaging

用于定量质谱成像的标记 IMS TAG 蛋白

• 批准号: NC/L001896/1
• 负责人: Malcolm Clench
• 金额: $ 30.88万
• 依托单位: Sheffield Hallam University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NC%2FL001896%2F1
• 关键词: Labelled; IMS; TAG; Proteins; Quantitative

This project aims to develop methodology for the quantitative Mass Spectrometry Imaging (MSI) of proteins in biological tissue. Two non-animal models will be used both of which comprise multi-cellular, three-dimensional (3D) tissue constructs fabricated from human cell lines; a novel type of tumour spheroid model developed in our laboratory and a commercial 3D human skin construct Successful development of the methodology will present an alternative to both in vivo and ex vivo testing using animal models to represent human tumours and skin. The developed methodology will also be applicable to animal tissue where the ability to image and quantify multiple non-labelled proteins has the potential to reduce the number of animals used in many procedures, in comparison to conventional techniques employing immunohistochemistry, and immunoflouresence.

该项目旨在开发生物组织中蛋白质的定量质谱成像 (MSI) 方法。将使用两种非动物模型，它们均包含由人类细胞系制造的多细胞三维（3D）组织结构；我们实验室开发的新型肿瘤球体模型和商业 3D 人体皮肤结构该方法的成功开发将为使用动物模型代表人类肿瘤和皮肤的体内和离体测试提供替代方案。所开发的方法也适用于动物组织，与采用免疫组织化学和免疫荧光的传统技术相比，对多种非标记蛋白质进行成像和定量的能力有可能减少许多程序中使用的动物数量。