MECHANISMS OF HISTAMINE RELEASE

组胺释放机制

• 批准号: 6161772
• 负责人: R P SIRAGANIAN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6161772
• 关键词: T cell receptor; antibody receptor; basophils; biological signal transduction; cell line; enzyme activity; histamine release; immunoregulation; inflammation; leukocyte activation /transformation; mast cell; neoplastic cell culture for noncancer research; phosphorylation; protein tyrosine kinase; secretion

The activation of mast cells and basophils by immunoglobulin cell surface receptors results in the release of an array of mediators. The goal of this project is to understand the mechanism of signal transduction from the immune receptors. The receptors on these cells are a model for studies of immunoglobulin receptors present on many immune cells such as B, T cells and monocytes. This project investigates the receptors and the molecular mechanisms involved in signal transduction in these cells. The model used for most of these studies is the rat basophilic leukemia RBL- 2H3 cell line. Goals are to characterize and identify the proteins which are activated in the signal cascade from the cell surface receptors that result in activation of cells to secrete granules. Emphasis has been on understanding the protein tyrosine phosphorylations that occur during cell activation, especially the activation of the protein kinase Syk. Studies identify the protein tyrosine kinases that are involved in these reactions together with their substrates. We are using immunological, biochemical, molecular and cell biological techniques.

免疫球蛋白细胞表面对肥大细胞和嗜碱性粒细胞的激活作用 受体导致一系列介质的释放。 的目标 本项目旨在了解信号转导的机制， 免疫受体。 这些细胞上的受体是 免疫球蛋白受体存在于许多免疫细胞上的研究， B、T细胞和单核细胞。 该项目研究受体和 参与这些细胞中信号转导的分子机制。 的 用于大多数这些研究的模型是大鼠嗜碱性白血病RBL， 2H3细胞株。 目标是表征和鉴定 在细胞表面受体的信号级联中被激活， 导致细胞活化以分泌颗粒。 重点是 了解蛋白质酪氨酸磷酸化，发生在细胞 活化，特别是蛋白激酶Syk的活化。 研究 确定参与这些反应的蛋白质酪氨酸激酶 以及它们的基底。 我们用免疫学，生化学， 分子和细胞生物学技术。