ROLE OF ADHESION MOLECULES IN MAST CELL AND BASOPHILS FUNCTION

粘附分子在肥大细胞和嗜碱性粒细胞功能中的作用

• 批准号: 6161856
• 负责人: R P SIRAGANIAN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6161856
• 关键词: basophils; biological signal transduction; cell adhesion molecules; extracellular matrix proteins; fibronectins; inflammation; integrins; mast cell; protein kinase; receptor binding; tissue /cell culture

The inflammatory response is characterized by the accumulation of extracellular matrix proteins at sites of injury. Mast cells and basophils accumulate at such inflammatory sites and play a role by releasing an array of mediators. Basophils and mast cells have surface adhesion receptors that are involved in the binding of these cells to other cells or to the extracellular matrix. Such integrin mediated adherence of cells to fibronectin stimulates cell spreading and generates intracellular signals. Previously we observed that the RBL-2H3 cells bind through integrin receptors to surfaces coated with fibronectin; this results in changes in the cytoskeleton, cell spreading and a redistribution of the granules to the periphery of the cells. These changes result in the tyrosine phosphorylation of several proteins including the focal adhesion kinase, pp125FAK (FAK) and in enhanced secretion by the cells. FAK is a widely expressed protein tyrosine kinase that localizes with integrins and several proteins to intracellular sites where cells are in contact with the extracellular matrix. FAK also becomes tyrosine phosphorylated in response to the activation of various receptors including FcepsilonRI and antigen receptors on T-cells. In studies during this year we have continued exploring the interaction of intracellular signals generated by adhesion and immune receptor activation.

炎症反应的特征是 细胞外基质蛋白在损伤部位。 肥大细胞和 嗜碱性粒细胞聚集在这些炎症部位， 释放出一系列的调解人 嗜碱性粒细胞和肥大细胞表面 粘附受体参与这些细胞与 其他细胞或细胞外基质。 这种整合素介导 细胞与纤连蛋白的粘附刺激细胞铺展并产生 细胞内信号 以前我们观察到RBL-2 H3细胞结合 通过整联蛋白受体与纤连蛋白包被的表面;这 导致细胞骨架的变化，细胞扩散和 颗粒重新分布到细胞的周边。 这些 变化导致几种蛋白质的酪氨酸磷酸化 包括粘着斑激酶pp 125 FAK（FAK）和增强的 由细胞分泌。 FAK是一种广泛表达的蛋白酪氨酸激酶 与整合素和几种蛋白质一起定位于细胞内位点 细胞与细胞外基质接触。 FAK也 酪氨酸磷酸化，以响应各种 受体，包括Fc ε RI和T细胞上的抗原受体。 在 在这一年的研究中，我们继续探索 粘附和免疫受体产生的细胞内信号 activation.