IN VITRO STUDIES OF SECRETORY CELL STRUCTURE AND FUNCTION

分泌细胞结构和功能的体外研究

• 批准号: 6161776
• 负责人: R P SIRAGANIAN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6161776
• 关键词: biological signal transduction; cell biology; histochemistry /cytochemistry; mast cell; receptor mediated endocytosis; secretion; structural biology; tissue /cell culture

The RBL-2H3 and other cultured cell lines are used to study various aspects of endocytic and secretory processes. Emphasis is on the use of morphological, cytochemical, biochemical characterization of cultured cells. Events involved in receptor activation and signal transduction are being investigated as well as endocytic and secretory pathways. Studies also investigate the cell biology of these cells, the pathways for their maturation, and development. During the last year antibodies have been identified that bind to mast cell precursors and these have been used to purify and identify these cells.

用RBL-2H3和其他培养的细胞系研究各种不同的 内吞和分泌过程的各个方面。重点是使用 培养物形态、细胞化学、生化特性的研究 细胞。参与受体激活和信号转导的事件有 正在被研究以及内吞和分泌途径。研究 也要研究这些细胞的细胞生物学，它们的途径 成熟和发展。在过去的一年里，抗体已经 确定了与肥大细胞前体结合，这些已被用于 纯化并鉴定这些细胞。