MECHANISMS OF HISTAMINE RELEASE

组胺释放机制

• 批准号: 6104526
• 负责人: R P SIRAGANIAN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6104526
• 关键词: T cell receptor; antibody receptor; basophils; biological signal transduction; cell line; enzyme activity; histamine release; immunoregulation; inflammation; leukocyte activation /transformation; mast cell; neoplastic cell culture for noncancer research; phosphorylation; protein tyrosine kinase; secretion

The activation of mast cells and basophils by immunoglobulin cell surface receptors results in the release of an array of mediators. This project investigates the receptors and the molecular mechanisms involved in signal transduction in these cells. The receptors on these cells are a model for studies of immunoglobulin receptors present on may immune cells such as B, T cells and monocytes. The goal of this project is to understand the mechanism of signal transduction from the immune receptors. The model used for most of these studies are the rat basophilic leukemia RBL-2H3 cells. Goals are to characterize and identify the proteins which are activated in the signal cascade from the cell surface receptors that result in activation of cells to secrete granules. Emphasis has been on understanding the protein tyrosine phosphorylations that occur during cell activation, especially the activation of the protein kinase Syk. Studies identify the protein tyrosine kinases that are involved in these reactions together with their substrates. We are using immunological, biochemical, molecular and cell biological techniques.

肥大细胞和嗜碱性粒细胞的激活 免疫球蛋白细胞表面受体导致释放一种 调解人的阵列。该项目研究受体和 参与这些细胞信号转导的分子机制。 这些细胞上的受体是研究 免疫球蛋白受体存在于免疫细胞上，如B， T细胞和单核细胞。本项目的目标是了解 免疫受体的信号转导机制。这个 这些研究中使用的大多数模型是大鼠嗜碱性粒细胞白血病 RBL-2H3细胞。目标是对蛋白质进行表征和鉴定 它们在来自细胞表面的信号级联中被激活 导致细胞激活以分泌颗粒的受体。 重点是了解蛋白质酪氨酸 在细胞激活过程中发生的磷酸化，尤其是 蛋白激酶Syk的激活。研究确定了这种蛋白质 参与这些反应的酪氨酸激酶与 它们的底物。我们使用的是免疫学，生化， 分子和细胞生物学技术。