GENERATION OF MOUSE MODELS OF NEUROLOGICAL DISORDERS

基因敲除小鼠模型已成为描绘 特定基因的分子和功能作用。为了试图 为神经系统疾病建立这样的模型，我们已经启动了 干扰小鼠胚胎载脂蛋白D(APOD)基因的研究 干细胞。我们用大鼠APOD基因探针对129/SVJ小鼠进行筛选 基因组文库。已分离出APOD的8个基因组克隆 特色化的。一种APOD靶向结构已经被设计用于 正/负选择。用RT-PCR方法克隆了APOD基因的全长序列 小鼠肾脏RNA。我们希望在未来生产一种APOD基因敲除小鼠 为了确定APOD在神经再生中的作用 以及它在乳腺癌和前列腺癌中的潜在作用。

Gene knock-out mouse models have become gold standards for delineating molecular and functional roles of specific genes. In an attempt to generate such models for neurological disorders, we have initiated studies to disrupt the apolipoprotein D (ApoD) gene in mouse embryonic stem cells. We have used rat ApoD cDNA probe to screen the 129/svj mouse genomic library. Eight genomic clones of ApoD have been isolated and characterized. An ApoD targeting construct has been engineered for positive/negative selection. ApoD cDNA has been cloned by RT-PCR from mouse kidney RNA. We expect to produce an ApoD knock-out mouse in the near future in order to determine the role of ApoD in nerve regeneration as well as its potential role in breast and prostate cancer.