Optimising liquid production of the insect pathogenic fungus Metarhizium anisopliae and enhancing the shelf life of blastospores.

优化昆虫病原真菌绿僵菌的液体生产并延长芽生孢子的保质期。

• 批准号: NE/H000321/1
• 负责人: Tariq Butt
• 金额: $ 9.96万
• 依托单位: Swansea University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FH000321%2F1
• 关键词: Optimising; liquid; production; insect; pathogenic

There is much interest in the development of insect pathogenic fungi like Metarhizium anisopliae as environmentally friendly alternatives to chemical pesticides. One of the major hurdles in the commercialisation of fungal biological control agents is production cost. An earlier 'Follow-On' NERC grant (NE/E522308/1) showed that M. anisopliae strains V275, 3297 and 4556 are highly stable (retaining their virulence upon repeated in vitro sub-culturing), and produce high yields of aerial conidia on broken rice. Recently, we found that these strains produced blastospores in liquid media. This creates the opportunity for mass production in liquid media which would reduce production costs further and generate highly competitive Metarhizium-based products. Blastospores are thin walled infection propagules which are less robust and have a shorter shelf life than aerial conidia (1-3 days versus several months at room temperature). The yield, virulence and shelf life of fungal propagules can be improved by manipulating nutrients and media water activity. The aims of this project are to develop media to (i) increase yields of M. anisopliae blastospores, (ii) demonstrate upscaled production without loss of quality and (iii) to develop formulations to enhance blastospore shelf life. Overall, the proposed work will reduce production costs and accelerate development of highly competitive commercial products based on M. anisopliae blastospores for control of a range of arthropod pests (e.g. vine weevil, thrips, ticks).

人们对开发像金龟子绿僵菌这样的昆虫病原真菌作为化学农药的环保替代品很感兴趣。真菌生物防治剂商业化的主要障碍之一是生产成本。 NERC 早期的“后续”资助 (NE/E522308/1) 显示 M. anisopliae 菌株 V275、3297 和 4556 高度稳定（在重复体外传代培养后保留其毒力），并在碎米上产生高产的气生分生孢子。最近，我们发现这些菌株在液体培养基中产生芽生孢子。这为液体培养基的大规模生产创造了机会，从而进一步降低生产成本并产生具有高度竞争力的绿僵菌产品。芽生孢子是薄壁的感染繁殖体，与气生分生孢子相比，其健壮性较差，保质期也较短（室温下为1-3天，而室温下为几个月）。真菌繁殖体的产量、毒力和保质期可以通过控制营养物质和介质水分活度来提高。该项目的目的是开发培养基，以（i）提高金龟子芽生孢子的产量，（ii）展示在不损失质量的情况下扩大生产规模，以及（iii）开发配方以延长芽生孢子的保质期。总体而言，拟议的工作将降低生产成本并加速开发基于金龟子芽生孢子的具有高度竞争力的商业产品，用于控制一系列节肢动物害虫（例如藤象甲、蓟马、蜱）。

项目成果

Differential susceptibility of Hyalomma excavatum adults and nymphs to the entomopathogens Metarhizium anisopliae ARSEF 4556 and Steinernema carpocapsae

• DOI: 10.1080/09670874.2016.1181287
• 发表时间: 2016-07-01
• 期刊: INTERNATIONAL JOURNAL OF PEST MANAGEMENT
• 影响因子: 1.5
• 作者: Butt, Tariq M.;Wood, Martyn;Hazir, Selcuk
• 通讯作者: Hazir, Selcuk