REGULATION OF VACCINIA VIRUS GENE EXPRESSION

痘苗病毒基因表达的调控

• 批准号: 6098907
• 负责人: BERNARD MOSS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6098907
• 关键词: Baculoviridae; electron microscopy; gene expression; gene targeting; genetic promoter element; genetic regulation; laboratory rabbit; open reading frames; recombinant DNA; recombinant virus; tissue /cell culture; transcription factor; vaccinia virus; virus genetics; virus infection mechanism

Poxviruses encode enzymes and factors needed for transcription of their genomes within the cytoplasm of infected cells. Vaccinia virus, the prototypic member of the poxvirus family, provides a unique system for combining biochemical and genetic approaches for investigating mechanisms of gene regulation and mRNA biosynthesis. Studies with vaccinia virus indicated that the genes are divided into three temporal classes - early, intermediate and late. Each gene class has a consensus DNA promoter sequence and corresponding transcription factors that interact with the virus-encoded multisubunit RNA polymerase. The transcription system for early genes is packaged within the infectious virus particle during its assembly, whereas the factors for intermediate and late gene transcription are synthesized successively after infection.

痘病毒编码所需的酶和因子