ALPHA HERPES VIRAL MEMBRANE PENETRATION GLYCOPROTEINS

α疱疹病毒膜穿透糖蛋白

• 批准号: 2757602
• 金额: $ 0.05万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2757602
• 关键词: biomedical resource; communicable diseases; microscopy; proteins; virus

My research focuses on the entry of herpes viruses into cells. Ten viral surface molecules interact with each other and with the cell surface to punch a hole into the cell. Individual molecules cannot be distinguished by the electron microscope and the complexes are too complicated to analyze by X-ray crystallography. I will propose to NIH on June 1 1993, that I make 2-D crystals of herpes viral membrane molecules alone and in combinations, image them at several angles in vitreous ice with low electron doses, and determine their 3-D structure by digitizing correlation and averaging, and Fourier analysis.

我的研究重点是疱疹病毒进入细胞。 十种病毒表面分子相互作用并与细胞相互作用 在细胞表面打孔。 单个分子不能 通过电子显微镜进行了表征， 很难用X射线晶体学分析 我会向你求婚 1993年6月1日，我制作了疱疹病毒膜的二维晶体， 分子单独和组合，图像他们在几个角度， 玻璃冰与低电子剂量，并确定其三维 结构的数字化相关和平均，和傅立叶 分析.