Flotillin link membrane microdomains to signalling endosome during T cell activation.

在 T 细胞激活过程中，Flotillin 将膜微结构域与信号内体连接起来。

• 批准号: DE140101626
• 负责人: Dr Jeremie Rossy
• 金额: $ 27.56万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Early Career Researcher Award
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DE140101626
• 关键词: Flotillin; link; membrane; microdomains; signalling

This project aims to determine the mechanisms that connect signalling microdomains at the cell surface to intracellular signalling endosomes to regulate T cell activation. A T cell immune response begins with the reorganisation of the plasma membrane to yield two-dimensional signalling microdomains that must be connected to the three-dimensional microarchitecture of the endocytic matrix for full T cell activation. This project hypothesises that Flotillin form distinct signalling microdomains in the plasma membrane that internalise to constitute an independent endocytic pathway. Using single-molecule and ultra-fast fluorescence imaging, the project will demonstrate that Flotillin represent a unique two-dimensional to three-dimensional regulatory mechanism for T cell signalling.

该项目旨在确定将细胞表面的信号微域连接到细胞内信号内体以调节T细胞激活的机制。T细胞免疫反应始于质膜的重组，以产生二维信号微域，这些微域必须与内吞基质的三维微结构相连，才能充分激活T细胞。这个项目假设Flotillin在质膜上形成不同的信号微域，这些微域内化构成一个独立的内吞途径。利用单分子和超快荧光成像，该项目将证明Flotillin代表了T细胞信号传递的独特的二维到三维调节机制。