MALDI ON MAGNETIC SECTOR BASED MASS SPECTROMETERS

基于磁扇区的质谱仪上的 MALDI

• 批准号: 6122180
• 负责人: RON ORLANDO
• 金额: $ 2.51万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6122180
• 关键词: biological products; biomedical equipment development; biomedical resource; spectrometry; technology /technique

We are developing new methods to obtain MALDI-MS and MALDI-MS/MS spectra on magnetic sector-based mass spectrometers. Our approach is based upon development of a new family of MALDI matrices. The novel feature of our new matrices is that they are viscous liquids and thus have self-healing surfaces. This property provides very long-lasting and reasonably constant ion currents from picomole quantities of analyte, allowing MALDI-magnetic sector spectra to be obtained even though a non-integrating point detector is used. This is the first time that MALDI has been successfully demonstrated on a sector-based MS without significant modification to the instrument.

我们正在开发新的方法来获得MALDI-MS和MALDI-MS/MS 基于磁扇区的质谱仪上的光谱。 我们的做法是 基于MALDI矩阵的新家族的开发。 小说 我们的新矩阵的特点是，它们是粘性液体， 有自我修复的表面。 这种特性提供了非常持久的 和合理恒定的离子电流从皮摩尔量的 分析物，允许MALDI磁扇区光谱获得，即使 尽管使用了非积分点检测器。 这是第一 MALDI在基于行业的 MS，未对仪器进行重大修改。