Photoreactive Self-Assembled Monolayers

描述（由申请人提供）：本项目旨在优化和 扩展了一期工程中展示的涂层技术， 其目的是提供一种简便、经济、适用范围广的薄膜 用于生物传感器和医疗器械表面钝化的涂层。 防止蛋白质和其他生物分子的非特异性结合是 对于各种生物材料、光学、电学 遭受污垢（蛋白质和细胞粘附， 微生物增殖和孔隙堵塞）与 生理液体和药物。一类新的嵌段共聚物 试剂制备和证明提供自组装单分子膜 其可以光化学固定在表面上。自发形成后 从水性涂布流体，疏水表面上的单层膜被 通过共价连接到表面和原位稳定 二嵌段聚合物分子的聚合或交联。所得 来自亲水性嵌段的“草地”抑制生物分子吸附， 可以为所需的生物分子如肝素提供附着位点。这 第二阶段的工作将综合改进的测试模型，这一类新的 多功能自组装单层分子。“活聚合” 将用于制备这些光反应性大分子单体表面活性剂， 在远端保护屏和血液透析膜上进行使用测试。 拟定商业应用： 这项工作预计将为远端的生物相容性提供新的试剂和涂层方法。 保护装置（血栓收集屏）和血液透析膜。 近100万患者每周需要进行三次血液透析。这些涂层 将为微孔医疗装置提供减少的结垢和增加的通量。 拟议的工作还将扩大嵌段共聚物技术， 用于增加润滑性的聚合物主链和用于 提高血液相容性，为各种医疗器械提供更好的涂层 装置.

DESCRIPTION (provided by applicant): This project is designed to optimize and extend the ultrathin coating technology demonstrated in the Phase I project, which is aimed at facile, cost-effective, and broadly applicable thin-film coatings for the passivation of biosensor and medical device surfaces. Prevention of non-specific binding of proteins and other biomolecules is important for a large variety of biomaterial, optical, electrical and structural surfaces which suffer fouling (protein and cellular adhesion, microbial proliferation, and pore plugging) from functioning in contact with physiological fluids and pharmaceuticals. A new class of block copolymer reagents was prepared and demonstrated to provide self-assembled monolayers which can be photochemically fixed on the surface. After spontaneous formation from aqueous coating fluid, the monolayer film on the hydrophobic surface is stabilized through covalent attachment to the surface and in situ polymerization or crosslinking of diblock polymer molecules. The resulting "field of grass" from the hydrophilic block inhibits biomolecule adsorption and can provide attachment sites for desired biomolecules such as heparin. This Phase II effort will synthesize improved test models of this new class of multifunctional self-assembling monolayer molecules. "Living polymerization" will be used to prepare these photoreactive macromer surfactants, which will be use-tested on distal protection screens and hemodialysis membranes. PROPOSED COMMERCIAL APPLICATION: This effort is expected to provide new reagents and coating methodology for distal protection devices (thrombi collection screens) and hemodialysis membranes. Almost one million patients need hemodialysis three times per week. These coatings would provide reduced fouling and increased flux for microporous medical devices. The proposed work will also extend the block copolymer technology to alternate polymer backbones for increased lubricity and to biomolecule immobilization for increased hemocompatibility, providing better coatings for a variety of medical devices.