PATTERNED IMMOBILIZATION OF BIOTIN ON GLASS

这项研究提出了一种方法来解决一个关键和持久的 开发可靠的生物传感器系统的障碍， 基因序列、癌症、传染性疾病 疾病和有毒物质。 这项研究的主要目标是 计划（SBIR阶段I，II和III）是开发和商业化 可用的、改进的多分析物生物传感器技术， 微观分析 该方法将涉及SurModics的扩展 PhotoLink技术一般分为表面钝化和 结合基团在传感器表面上的图案化固定。 的 模型传感器表面将采用两亲聚合物进行玻璃钝化 或蛋白质，并用广泛使用的生物素-抗生物素蛋白结合 汇率 生物素将（1）共价结合到钝化表面 通过掩模照射以光化学方式形成200 μ m的点，（2） 饱和的高亲和力多位点结合蛋白X- 抗生物素蛋白（抗生物素蛋白、中性抗生物素蛋白TM或链霉抗生物素蛋白）通过亲和结合 粗源材料，和（3）通过负载的固定化X-抗生物素蛋白点， 用所需的生物素化的寡核苷酸进行喷墨印刷， 耐抗生素结核分枝杆菌 检测/识别。 因此，这种方法提出了一种创新的 双自由基光化学和高亲和力特异性 结合对，以提供成本效益高的涂层技术， 图案化的多分析物生物传感器表面。 建议的商业应用 这项合成工作预计将在第一阶段产生两个新的 供研究和工业界使用的适销产品： (1)光反应性玻璃/硅片和（2）钝化生物素化 玻璃/硅晶片。 生产具有成本效益的 生物分子相容的图案化多分析物传感器表面， 在第二阶段证明，具有公认的大价值的生物传感器 行业

This research presents an approach to resolving a critical and enduring obstacle to developing dependable biosensor systems for the timely measure of multiple markers for gene sequences, cancers, infectious diseases, and toxic agents. The broad goal of this proposed research program (SBIR Phases I, II and III) is to develop and make commercially available, improved multianalyte biosensor technology for health-related microanalyses. The approach will involve the extension of SurModics PhotoLink technology into general passivation of the surface and patterned immobilization of binding groups on the sensor surface. The model sensor surface will be glass passivated with amphiphilic polymers or proteins and patterned with the widely useful biotin--avidin binding pair. Biotin will be (1) covalently bound to the passivated surface photochemically in 200 mum dots by illumination through a mask, (2) saturated with its very high affinity, multi-site binding protein X- avidin (avidin, NeutravidinTM, or streptavidin) by affinity binding from crude source material, and (3) the immobilized X-avidin dots loaded by ink-jet printing with the desired biotinylated oligonucleotides for antibiotic resistant Mycobacterium tuberculosis (TB) detection/identification. This approach thus present an innovative combination of diradical photochemistry and high-affinity specific binding pairs to provide a cost-effective coating technology for patterned multianalyte biosensor surfaces. PROPOSED COMMERCIAL APPLICATION This synthetic work is expected to generate in Phase I, two new marketable products for use by the research and industrial communities: (1) Photoreactive glass/silicon wafers and (2) Passivated biotinylated glass/silicon wafers. The potential for cost-effective production of biomolecule-compatible patterned multianlyte sensor surfaces, to be demonstrated in Phase II, has a recognized large value to the biosensor industry.