15N LABELING OF ANTI HIV I RIBOZYME

抗 HIV I 核酶的 15N 标记

• 批准号: 6309036
• 负责人: RAMASWAMY H SARMA
• 金额: $ 2.74万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2002-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6309036
• 关键词: AIDS; biomedical resource; immunology; infection; inflammation; lymphatic system; nucleic acids; virus

The SIR provided 13C, "N DNA; 50mg Due to their ability ot specifically recognize targeted sequences through Watson-Crick base pairing, anti-sese NDA/RNA have been shown to be an effective tool in distrpting the life cycle of a virus. Recently it has been shown that the incorporationo f catalytic RNA species derived from small plant pathogenic RNA's further enhanced the therapeutic ability of anti-sense oligonucleotides by overcoming its stoichiometrical requirement. We are studying the abortive complex between a 30-mer ribozyme and a 17-mer DNA analog of HIV- I GAG-RNA. Using uniformly labeled DNA we will employ 3 and 4-D NMR techniques to determine the structure.

SIR提供了13 C，N DNA; 50 mg由于它们的能力， 通过沃森-克里克碱基特异识别靶序列 配对，抗sese NDA/RNA已被证明是一种有效的工具， 破坏了病毒的生命周期 最近的研究表明， 小型植物中催化RNA种类分离 病原性RNA进一步增强了 反义寡核苷酸通过克服其化学计量 要求。 我们正在研究一个30聚体 核酶和HIV-1GAG-RNA的17-mer DNA类似物。 统一使用 标记的DNA，我们将采用3和4-D NMR技术来确定 结构