15N LABELING OF ANTI HIV I RIBOZYME

SIR提供了~(13)C，“N DNA；50毫克，因为他们有能力 通过Watson-Crick碱基特异性识别靶序列 配对、抗Sese的NDA/RNA已被证明是一种有效的工具 分散了病毒的生命周期。最近有证据表明， 小植物来源的催化RNA物种的掺入 致病核糖核酸的进一步增强了对白血病的治疗能力 克服化学计量学的反义寡核苷酸 要求。我们正在研究一个30多岁的人 核酶和HIV-I Gag-RNA的17聚体DNA类似物。统一使用 我们将使用三维和四维核磁共振技术来确定标记的DNA 结构。

The SIR provided 13C, "N DNA; 50mg Due to their ability ot specifically recognize targeted sequences through Watson-Crick base pairing, anti-sese NDA/RNA have been shown to be an effective tool in distrpting the life cycle of a virus. Recently it has been shown that the incorporationo f catalytic RNA species derived from small plant pathogenic RNA's further enhanced the therapeutic ability of anti-sense oligonucleotides by overcoming its stoichiometrical requirement. We are studying the abortive complex between a 30-mer ribozyme and a 17-mer DNA analog of HIV- I GAG-RNA. Using uniformly labeled DNA we will employ 3 and 4-D NMR techniques to determine the structure.