DETERMINATION OF GLYCOSYLATION SITES OF GLYCOPROTEINS

我们开发了一种新的方法， 解吸/电离质谱（MALDI-MS）和串联MALDI （MALDI-MS/MS）谱图将在基于磁扇区的质谱上获得 光谱仪 我们的方法是基于一个新的家庭的发展 MALDI矩阵。 新矩阵的新颖特征在于， 是粘性液体，因此具有自我修复的表面。 这 性能提供了一个持久的和合理的恒定的离子流 来自皮摩尔量的分析物的电流。 MALDI-磁扇区 因此，即使非积分，也可以获得光谱。 使用点检测器。 这是第一次，MALDI已经 在基于扇区的MS上演示，无需对 工具。 能够在基于扇区的 仪器预计将在我们的项目中发挥关键作用， 寡糖碱MS/MS分析 金属加合物。 因为MALDI是 比快原子轰击（FAB）灵敏，样品要求 所提出的分析方案显著减少。 的能力 通过MALDI分析这些样品，也使我们提出的方法 对样品中碱金属盐的量不太敏感， 极大地促进了我们的MS/MS研究。

We have developed a new method that permits matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and tandem MALDI (MALDI-MS/MS) spectra to be obtained on magnetic sector-based mass spectrometers. Our approach is based upon development of a new family of MALDI matrices. The novel feature of the new matrices is that they are viscous liquids and thus have self-healing surfaces. This property provides a long-lasting and reasonably constant flow of ion currents from picomole quantities of analyte. MALDI-magnetic sector spectra can be obtained, therefore, even though a non-integrating point detector is used. This is the first time that MALDI has been demonstrated on a sector-based MS without significant modification to the instrument. The ability to perform MALDI on sector-based instruments is expected to play a key role in our project involving the structural analysis of oligosaccharides by MS/MS of their alkali metal adducts. Because MALDI is approximately 1,000 times more sensitive than fast atom bombardment (FAB), sample requirements for the proposed analytical scheme are significantly reduced. The ability to analyze these samples by MALDI has also made our proposed approach much less sensitive to the amount of alkali salt in the sample, greatly facilitating our MS/MS study.