Topical 'photo-chemoprevention' of oral leukoplakia

描述（由申请人提供）： 流行病学研究支持高摄入量 水果和蔬菜和低癌症风险。 长期目标是 本项目旨在研究果实色素，特别是花青素， 人类的癌症 花色苷是一类普通黄酮类化合物， 化合物，其特征在于黄环。 浆果是 世界上广泛食用的典型水果，有暗红色，紫色和蓝色 颜色，并富含花青素。 我们假设花青素- 丰富的浆果可能在预防癌症方面发挥重要作用。 几乎没有信息是关于癌症的化学预防特性， 浆果，包括标准化，安全性，生物利用度和生物活性。 这一提议将为更广泛的化学预防研究奠定基础 浆果。 天然花青素有7000多种。 花青素是一种典型的 花青素具有红色，出现在大多数浆果中。 黑樱桃是一种 黑果腺肋花楸属的美洲本土浆果。 相比其他 浆果中，它含有较多的花青苷，约占总花青苷的96%。在 本建议我们将重点放在标准化的化学预防作用， 用一种新的方法， 3-三维重建的正常人上皮细胞培养模型，如 正常人表皮角质形成细胞（NHEK）和正常人支气管 上皮（NHBE）。 该提案的三个具体目标是：1）安全 SECK在3-D NHEK和NHTBE中的评价; 2）SECK在3-d中的生物利用度 NHEK和NHBE以及3）SECK对苯并（a）芘诱导的3-D细胞凋亡的影响 NHEK和NHBE。 这项试验性研究将提供更多有价值的信息， 未来的体内临床前和临床研究。 我们的3D重组人 正常上皮细胞培养模型也可以提供快速和可靠的模型 用于筛选化学预防植物药。

DESCRIPTION (provided by applicant): Epidemiological studies support the association between the high intake of fruits and vegetables and the low risk of cancer. The long-term goal of this project is to investigate if fruit pigment, especially, anthocyanins, prevent cancer in humans. Anthocyanins are a class of the general flavonoid group of compounds, which are characterized by the flavylium nucleus. Berries are typical fruits widely consumed in the world with dark red, purple and blue colors, and is rich in anthocyanins. It is our hypothesis that anthocyanin- rich berries may play an important contribution in the cancer prevention. Little information is available on the cancer chemoprevention properties of berries, including standardization, safety, bioavailability and bioactivity. This proposal would support a basis for more extended chemoprevention research of berries. There are more 7000 natural anthocyanins. Cyanin is a typical anthocyanin with red color and occurs in most berries. Black chokeberry is a native American berry of the Aronia melanocarpa species. Compared to other berries, it contains more cyanins, about 96% of the total anthocyanins. In this proposal we will focus on the chemopreventive effect of standardized extract of chokeberry (SECK) and their major cyanin constituents using a novel 3-dimensional reconstituted normal human epithelium culture model, such as normal human epidermal keratinocytes (NHEK) and normal human bronchial epithelium (NHBE). Three Specific Aims of this proposal are: 1) Safety evaluation of SECK in 3-D NHEK and NHTBE; 2) Bioavailability of SECK in 3-d NHEK and NHBE and 3) Effect of SECK on benzo(a)pyrene-induced apoptosis in 3-D NHEK and NHBE. This pilot study will provide more valuable information for future in vivo preclinical and clinical studies. Our 3-D reconstituted human normal epithelium culture models may also provide a rapid and reliable model for screening chemopreventive botanicals.