Topical 'photo-chemoprevention' of oral leukoplakia

描述(由申请人提供)： 流行病学研究支持高摄入量与 水果和蔬菜以及患癌症的低风险。这样做的长期目标是 项目是研究水果色素，特别是花青素，是否能防止 人类的癌症。花青素是黄酮类植物中的一类。 化合物，其特征是黄色素核。浆果是 世界上广泛食用的典型水果，有深红色、紫色和蓝色 色泽鲜艳，富含花青素。我们的假设是花青素- 丰富的浆果可能在预防癌症方面发挥重要作用。 关于黄曲霉毒素的抗癌作用的信息很少。 浆果，包括标准化、安全性、生物利用度和生物活性。 这项提议将为更广泛的化学预防研究奠定基础。 浆果的味道。天然花青素有7000多种。蓝蛋白是一种典型的 花青素呈红色，存在于大多数浆果中。黑木莓是一种 黑果天牛属植物的美洲原住民浆果。与其他 浆果中，它含有较多的花青素，约占总花青素的96%。在……里面 这个建议我们将重点放在标准化的化学预防作用上 一种新型的木莓提取物(SECK)及其主要蓝蛋白成分 三维重组正常人上皮细胞培养模型 正常人表皮角质形成细胞(NHEK)和正常人支气管 上皮细胞(NHBE)。这项提议的三个具体目标是：1)安全 SECK在三维NHEK和NHTBE中的评价2)SECK的三维生物利用度 NHEK和NHBE以及3)Seck对苯并(A)芘诱导的三维细胞凋亡的影响 Nhek和NHBe。这项初步研究将为以下方面提供更有价值的信息 未来的体内临床前和临床研究。我们的3-D重组人 正常的上皮细胞培养模型也可能提供一种快速可靠的模型。 用于筛选化学预防植物类药物。

DESCRIPTION (provided by applicant): Epidemiological studies support the association between the high intake of fruits and vegetables and the low risk of cancer. The long-term goal of this project is to investigate if fruit pigment, especially, anthocyanins, prevent cancer in humans. Anthocyanins are a class of the general flavonoid group of compounds, which are characterized by the flavylium nucleus. Berries are typical fruits widely consumed in the world with dark red, purple and blue colors, and is rich in anthocyanins. It is our hypothesis that anthocyanin- rich berries may play an important contribution in the cancer prevention. Little information is available on the cancer chemoprevention properties of berries, including standardization, safety, bioavailability and bioactivity. This proposal would support a basis for more extended chemoprevention research of berries. There are more 7000 natural anthocyanins. Cyanin is a typical anthocyanin with red color and occurs in most berries. Black chokeberry is a native American berry of the Aronia melanocarpa species. Compared to other berries, it contains more cyanins, about 96% of the total anthocyanins. In this proposal we will focus on the chemopreventive effect of standardized extract of chokeberry (SECK) and their major cyanin constituents using a novel 3-dimensional reconstituted normal human epithelium culture model, such as normal human epidermal keratinocytes (NHEK) and normal human bronchial epithelium (NHBE). Three Specific Aims of this proposal are: 1) Safety evaluation of SECK in 3-D NHEK and NHTBE; 2) Bioavailability of SECK in 3-d NHEK and NHBE and 3) Effect of SECK on benzo(a)pyrene-induced apoptosis in 3-D NHEK and NHBE. This pilot study will provide more valuable information for future in vivo preclinical and clinical studies. Our 3-D reconstituted human normal epithelium culture models may also provide a rapid and reliable model for screening chemopreventive botanicals.