METALLOREGULATION OF HEAVY METAL RESISTANCE GENES IN BACTERIA

细菌中重金属抗性基因的金属调控

• 批准号: 6437595
• 负责人: ROBERT A SCOTT
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437595
• 关键词: bacteria; biomaterials; biomedical resource; genome; proteins; spectrometry; structural biology

We propose to study the interaction of metals with proteins involved in regulated heavy metal resistance in bacteria. We have chosen two well-studied examples: the gene products of the mer operon from Gram negative transposon Tn21 that confers mercury resistance; and the gene products of the ars operon from E. coli plasmid R773 that confers arsenical and antimonial resistance. In the mer operon, Hg(II), Cd(II), and Zn(II) interactions with the MerR metalloregulatory protein and mutants that have altered metal activation specificities will be compared at the level of active-site structure. In the ars operon, As(III) and Sb(III) binding sites on ArsR and ArsD, two co-regulatory proteins that control ars gene expression by an arsenite/antimonite-dependent mechanism, and ArsA, the ATP-dependent and M(III)-activated arsenite/antimonite ion transporter, will be defined. Site-directed mutagenesis will be used to determine the residues involved in As(III) and Sb(III) binding sites.

我们建议研究金属与蛋白质的相互作用。 参与调节细菌对重金属的抗性。我们有 选择了两个研究得很好的例子：分子操纵子的基因产物 来自具有汞抗性的革兰氏阴性转座子TN21； 和来自大肠杆菌R773的Ars操纵子的基因产物 赋予阿森纳和锑的抗药性。在梅尔歌剧中， 汞(II)、镉(II)和锌(II)与MerR的相互作用 金属调节蛋白和改变金属的突变体 激活特性将在活动站点级别进行比较 结构。在Ars操纵子中，As(III)和Sb(III)结合部位 ArsR和ARSD--两种控制Ars基因的共调控蛋白 通过亚砷酸盐/锑依赖机制表达，以及ARSA， 三磷酸腺苷依赖和M(III)激活的亚砷/锑离子 运输者，将被定义。将使用定点突变 测定As(III)和Sb(III)结合所涉及的残留物 网站。