XRAY SCATTERING ON MG2+ PROMOTED STRUCT CHANGE OF ENDO & EXONUCLEASE FEN 1

MG2上的X射线散射促进了ENDO的结构变化

• 批准号: 6437699
• 负责人: HIROTSUGU TSURUTA
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437699
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; genome; proteins; radiography; structural biology

This 42 kDa endo-/exonuclease (flap endonuclease and 5'-exonuclease) FEN-1 is important in DNA replication and damage repair. We conducted preliminary solution scattering experiments in the presence and absence of Mg2+ on the wild type FEN-1 and a site-directed mutant whose repair function is inactivated. Although the high-flux synchrotron beam allowed us to study these proteins at fairly low concentrations (as low as 1 mg/ml), our preliminary results suggest that the proteins needs to be measured at a rather low temperature (around 5 0C) instead of 15 0C where most of the preliminary measurements were done this time. Further studies will be performed by submitting a separate experimental proposal. Parallel neutron scattering studies are in progress.

这种42 kDa内切核酸酶/外切核酸酶（瓣状核酸内切酶和 FEN-1在DNA复制和损伤中起重要作用 修复. 我们进行了初步的溶液散射实验， 在野生型FEN-1上存在和不存在Mg 2+， 修复功能失活的定点突变体。 虽然 高通量同步加速器光束使我们能够研究这些蛋白质， 相当低的浓度（低至1 mg/ml），我们的初步结果 这表明蛋白质需要在相当低的温度下测量， 温度（约5摄氏度），而不是15摄氏度，其中大部分 这次进行了初步测量。 进一步的研究将 通过提交单独的实验提案进行。 平行 中子散射研究正在进行中。