C. elegans models for human transport disorders

人类运输障碍的线虫模型

• 批准号: 6440275
• 负责人: Colin Manoil
• 金额: $ 14.94万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-24 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440275
• 关键词: Caenorhabditis elegans; fluorescence microscopy; gene expression; gene induction /repression; gene mutation; genetic models; genetically modified animals; green fluorescent proteins; model design /development; polymerase chain reaction; transport proteins

DESCRIPTION (provided by applicant): The goal of the proposed research is to develop one or more C. elegans genetic models for intestine and kidney transporter defects. We have identified eight nematode homologues of transporters directly implicated in digestive and renal disease or otherwise thought to be important in the functions of these organs. In this project, we propose to characterize the expression patterns of the nematode proteins and determine the properties of animals defective in their functions. Based on this information, stable loss of function mutations will be sought for those genes judged to be the most promising candidates for transporter disease models.

描述（由申请人提供）： 本研究的目标是开发一种或多种C.线虫遗传 肠和肾转运蛋白缺陷的模型。我们已经确认了八个 直接参与消化和肾脏转运蛋白的线虫同源物 疾病或以其他方式被认为是重要的功能，这些器官。 在这个项目中，我们提出了表征的表达模式， 线虫蛋白质，并确定动物的属性有缺陷的， 功能协调发展的基于这一信息，稳定的功能丧失突变将被发现。 寻找那些被认为是最有希望的候选基因， 转运蛋白疾病模型。