Bacterial Mutant Library Core

细菌突变体库核心

• 批准号: 8236990
• 负责人: Colin Manoil
• 金额: $ 21.86万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8236990
• 关键词: Achievement; Alleles; Antibiotics; Burkholderia pseudomallei; Centers of Research Excellence; DNA Resequencing; Emerging Communicable Diseases; Francisella; Francisella tularensis; Gene Expression; Gene Fusion; Generations; Genes; Genetic; Genome; Goals; Growth; Homologous Gene; Insertion Mutation; Laboratories; LacZ Genes; Libraries; Methods; Mutagenesis; Mutation; Pathogenesis; Phenotype; Plasmids; Procedures; Quality Control; Reporter; Resources; Site; Source; Testing; Virulence; Virulent; Work; Yersinia pestis; biodefense; mutant; promoter

The main goal of the Core is to generate near-saturation sequence-defined transposon mutant libraries of Yersinia pestis and Burkholderia pseudomallei. The libraries should include mutants with insertions in nearly all genes nonessential for growth on laboratory medium. Mutants from the high coverage libraries (& 5 unique insertions/gene) produced initially will be used to create smaller libraries providing relatively complete, redundant (two insertions/gene) coverage of the nonessential genome. The transposons used for mutagenesis will generate lacZ gene fusions, and the libraries will thus serve as sources of both insertion mutations and reporter fusions. The Core will also work to develop methods to transfer transposon mutations from a previously completed Francisella novicida library to Francisella holarctica FSC200 to facilitate genetic studies of a highly virulent Francisella strain.

核心的主要目标是生成近饱和序列定义的转座子突变文库