Bacterial Mutant Library Core

核心的主要目标是产生近饱和的序列定义的转座子突变体库， 鼠疫耶尔森菌和类鼻疽伯克霍尔德菌。文库应包括在近100个碱基中插入的突变体。 所有基因对实验室培养基上的生长都是非必需的。来自高覆盖文库的突变体（&5 独特的插入/基因）将用于创建较小的文库， 非必需基因组的完全冗余（两个插入/基因）覆盖。转座子用于 诱变将产生lacZ基因融合体，因此文库将作为两种插入的来源 突变和报告基因融合。核心还将致力于开发转移转座子的方法 从先前完成的新杀弗朗西斯菌文库到全北极弗朗西斯菌FSC 200的突变， 促进了对高毒力弗朗西斯菌菌株的遗传研究。

The main goal of the Core is to generate near-saturation sequence-defined transposon mutant libraries of Yersinia pestis and Burkholderia pseudomallei. The libraries should include mutants with insertions in nearly all genes nonessential for growth on laboratory medium. Mutants from the high coverage libraries (& 5 unique insertions/gene) produced initially will be used to create smaller libraries providing relatively complete, redundant (two insertions/gene) coverage of the nonessential genome. The transposons used for mutagenesis will generate lacZ gene fusions, and the libraries will thus serve as sources of both insertion mutations and reporter fusions. The Core will also work to develop methods to transfer transposon mutations from a previously completed Francisella novicida library to Francisella holarctica FSC200 to facilitate genetic studies of a highly virulent Francisella strain.