Bacterial Mutant Library Core

核心的主要目标是产生近饱和序列定义的转座子突变文库 鼠疫耶尔森氏菌和假腮腺伯克霍尔德氏菌。文库应该包括插入到近 在实验室培养基上对生长不必要的所有基因。来自高覆盖率文库的突变体(&5 最初产生的独特插入/基因)将用于创建较小的文库，提供相对 对非必需基因组的完全的、多余的(两个插入/基因)覆盖。转座子用于 突变将产生LacZ基因融合，因此文库将作为两种插入的来源 突变和记者融合。CORE还将致力于开发转移转座子的方法 从之前已完成的诺维氏链球菌文库到霍氏链球菌FSC200的突变 促进一种高毒力弗朗西斯拉菌株的基因研究。

The main goal of the Core is to generate near-saturation sequence-defined transposon mutant libraries of Yersinia pestis and Burkholderia pseudomallei. The libraries should include mutants with insertions in nearly all genes nonessential for growth on laboratory medium. Mutants from the high coverage libraries (& 5 unique insertions/gene) produced initially will be used to create smaller libraries providing relatively complete, redundant (two insertions/gene) coverage of the nonessential genome. The transposons used for mutagenesis will generate lacZ gene fusions, and the libraries will thus serve as sources of both insertion mutations and reporter fusions. The Core will also work to develop methods to transfer transposon mutations from a previously completed Francisella novicida library to Francisella holarctica FSC200 to facilitate genetic studies of a highly virulent Francisella strain.