Bacterial Phenotypic Diversity

细菌表型多样性

• 批准号: 7675874
• 负责人: Colin Manoil
• 金额: $ 28.58万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-20 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7675874
• 关键词: Achievement; Alleles; Anti-Bacterial Agents; Antibiotic Resistance; Antibiotics; Base Sequence; Biological Assay; Burkholderia pseudomallei; Centers of Research Excellence; Databases; Diagnostic; Diagnostic tests; Disease; Drug Delivery Systems; Emerging Communicable Diseases; Exhibits; Generations; Genes; Genetic; Genome; Genomics; Goals; Growth; Image Analysis; Infection; Laboratories; Libraries; Mediating; Melioidosis; Methods; Microarray Analysis; Mus; Mutation; Organism; Pathogenesis; Phenotype; Physiological; Procedures; Property; Regulation; Research; Resistance; Screening procedure; Stress; Technology; Testing; Variant; Virulence; Work; base; biodefense; biological adaptation to stress; clinically relevant; insight; mutant; new technology; novel; research study; stress tolerance; technology development; trait

The primary goal of the research is to identify the FT and BP genes responsible for several intrinsic antibiotic resistance, virulence and stress tolerance traits. The results should provide mechanistic insights into the physiological basis of each and may identify potential antibacterial drug targets. An additional set of experiments will help define BP species diversity in these and other phenotypes, and may identify diagnostic tests to differentiate natural isolates. As part of the project, a new-generation sequencing-based technology for assessing the makeup of transposon mutant pools of will be developed.

该研究的主要目标是确定负责几种内在抗生素的FT和BP基因 抗性、毒力和耐逆性状。这些结果应该提供机械的见解， 生理基础，并可能确定潜在的抗菌药物的目标。附加的一组 实验将有助于确定BP物种多样性在这些和其他表型，并可能确定诊断 测试以区分自然分离物。作为该项目的一部分，新一代测序技术 用于评估转座子突变库的组成。