Bacterial Phenotypic Diversity

细菌表型多样性

• 批准号: 8236984
• 负责人: Colin Manoil
• 金额: $ 29.29万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8236984
• 关键词: Achievement; Anti-Bacterial Agents; Antibiotic Resistance; Base Sequence; Burkholderia pseudomallei; Centers of Research Excellence; Databases; Diagnostic; Diagnostic tests; Drug Delivery Systems; Emerging Communicable Diseases; Generations; Genes; Goals; Growth; Libraries; Phenotype; Physiological; Research; Resistance; Stress; Technology; Testing; Variant; Virulence; base; biodefense; insight; mutant; research study; stress tolerance; trait

The primary goal of the research is to identify the FT and BP genes responsible for several intrinsic antibiotic resistance, virulence and stress tolerance traits. The results should provide mechanistic insights into the physiological basis of each and may identify potential antibacterial drug targets. An additional set of experiments will help define BP species diversity in these and other phenotypes, and may identify diagnostic tests to differentiate natural isolates. As part of the project, a new-generation sequencing-based technology for assessing the makeup of transposon mutant pools of will be developed.

该研究的主要目标是确定负责几种内在抗生素的 FT 和 BP 基因 抗性、毒力和应激耐受性特征。结果应提供有关机制的见解 每个的生理学基础并可以确定潜在的抗菌药物靶点。额外的一组 实验将有助于定义这些和其他表型的 BP 物种多样性，并可能确定诊断 区分天然分离株的测试。作为该项目的一部分，新一代基于测序的技术 将开发用于评估转座子突变体库的组成。