Assigning function of unknown genes of Acinetobacter baumanii by use of genetic

项目1将识别不动杆菌中功能未知的保守基因并分配功能 鲍曼不动杆菌，一种引起医院获得性抗生素耐药性感染的新兴病原体。项目 将集中在蛋白质编码基因，有助于任何七个临床相关的阻力性状。我们 假设这些基因控制外排泵的表达和活性、膜通透性 决定因素、失活酶和应激反应。对四种抗生素产生耐药性所需的基因， 两种杀虫剂和干燥剂将首先通过使用Tn-seq的基因组规模的突变体筛选来鉴定 技术.然后，使用一组组合来表征未知功能的抗性基因的子集 基因组学和蛋白质组学的方法。结果将在当前的背景下解释， 了解每一个性状，以便为所研究的基因制定潜在的分子功能。 然后将使用针对单个基因的方法来测试假设的功能。

Project 1 will identify and assign functions to conserved genes of unknown function in Acinetobacter baumannii, an emerging pathogen that causes hospital-acquired, antibiotic resistant infections. The project will focus on protein-coding genes that contribute to any of seven clinically relevant resistance traits. We hypothesize that such genes control the expression and activities of efflux pumps, membrane permeability determinants, inactivating enzymes, and stress responses. Genes required for resistance to four antibiotics, two biocides and desiccation will first be identified by genome-scale mutant screening using Tn-seq technology. A subset of the resistance genes of unknown function will then be characterized using a battery of genetic, genomic and proteomic approaches. The results will be interpreted in the context of current understanding of each trait in order to formulate potential molecular functions for the genes examined. Hypothesized functions will then be tested using approaches tailored to individual genes.