IMAGING VIRUS PARTICLES W/ 300 KEV ELECTRON CRYOMICROSCOPE

该项目旨在评估300keV电子伏特的潜力 带场发射枪和液氦的电子冷冻显微镜 冷冻阶段。这样的仪器只在日本有售。我们僵住了 在休斯顿EM网格上的P22噬菌体样本，并将它们运往 液氮送往日本的S博士实验室。乔安妮塔 Jakana和Wah Chiu在日本进行了成像。几套 经过一周的紧张工作，获得了高质量的图像。我们的 随后的计算机分析表明， 在日本拍摄的图像的计算转换不像 这是在休斯顿用等值散焦拍摄的400keV图像。 这些初步数据已经提供了开发一种 向NSF提交的购买此类工具的拨款提案， 在美国，它的价格约为200万美元。我们已经获得了一个强有力的 贝勒S政府承诺配套资金支持这一项目 批准申请。NSF提供100万美元资金的通知刚刚发出 已于今日收到。

This project is aimed at evaluating the potential of a 300 keV electron cryomicroscope with a field emission gun and a liquid helium cryostage. Such an instrument is only available in Japan. We froze samples of P22 phage on EM grids in Houston and shipped them under liquid nitrogen to Dr. Y. Fujiyoshi s laboratory in Japan. Joanita Jakana and Wah Chiu performed the imaging in Japan. A few sets of quality images were obtained in a week of intensive work. Our subsequent computer analysis indicated that the damping of the computed transform of the images taken in Japan was not as steep as that of the 400 keV images taken with equivalent defocus in Houston. This preliminary data has provided the results needed to develop a grant proposal submitted to NSF for acquisition of such an instrument, which costs ~US$2 million, in the US. We have obtained a strong matching fund commitment from Baylor s administration to support this grant application. Notice of funding of $ 1 million from NSF has just been received today.