IMAGING VIRUS PARTICLES W/ 300 KEV ELECTRON CRYOMICROSCOPE

使用 300 KEV 电子低温显微镜对病毒颗粒进行成像

• 批准号: 6504536
• 负责人: Wah Chiu
• 金额: $ 13.47万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6504536
• 关键词: biomedical resource; infection; technology /technique; virus

This project is aimed at evaluating the potential of a 300 keV electron cryomicroscope with a field emission gun and a liquid helium cryostage. Such an instrument is only available in Japan. We froze samples of P22 phage on EM grids in Houston and shipped them under liquid nitrogen to Dr. Y. Fujiyoshi s laboratory in Japan. Joanita Jakana and Wah Chiu performed the imaging in Japan. A few sets of quality images were obtained in a week of intensive work. Our subsequent computer analysis indicated that the damping of the computed transform of the images taken in Japan was not as steep as that of the 400 keV images taken with equivalent defocus in Houston. This preliminary data has provided the results needed to develop a grant proposal submitted to NSF for acquisition of such an instrument, which costs ~US$2 million, in the US. We have obtained a strong matching fund commitment from Baylor s administration to support this grant application. Notice of funding of $ 1 million from NSF has just been received today.

该项目旨在评估 300 keV 的潜力 配有场发射枪和液氦的电子冷冻显微镜 低温阶段。 这种仪器仅在日本有售。 我们愣住了 休斯顿 EM 网格上的 P22 噬菌体样本，并通过 液氮送往日本 Y. Fujiyoshi 博士的实验室。 乔安妮塔 Jakana 和 Wah Chiu 在日本进行了成像。 几套 经过一周的紧张工作，获得了高质量的图像。 我们的 随后的计算机分析表明，阻尼 在日本拍摄的图像的计算变换并不像 与在休斯顿使用同等散焦拍摄的 400 keV 图像的结果相同。 这些初步数据提供了开发 向 NSF 提交购买此类仪器的拨款提案， 在美国花费约 200 万美元。 我们已经获得了强大的 贝勒政府承诺提供配套资金来支持这一目标 拨款申请。 刚刚收到 NSF 100 万美元资助的通知 今天已收到。