CORE--MASS SPECTROMETRY LABORATORY

核心--质谱实验室

• 批准号: 6500420
• 负责人: VERNON R YOUNG
• 金额: $ 10.37万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6500420
• 关键词: biomedical facility; mass spectrometry; nutrition; nutrition related tag; radiotracer

Mass spectrometric techniques, particularly those requiring the measurement of stable (heavy) nuclide enrichment in biological matrices have become essential tools in the study of nutrition and metabolism under various pathophysiological conditions. The nutrition/metabolic research program within the CNRU has given particular impetus to this area over the past years and it continues to be directed towards a better definition and characterization of the changes in nutrient, fuel and substrate metabolism that accompany various pathophysiological states. A part-time manager will support this core facility together with a well trained full-time technician. The principal functions of the mass spectrometry facility are four: 1. To provide timely and accurate determinations of stable isotope enrichments of tracers and their derivatives in bodily fluids, tissues, and expired air. 2. To assist in development of new methods for isotope analyses of compounds in blood tissues, and urine, as required to meet the needs of the investigators who are participating in the Center Grant research program. 3. To provide quality control for all analysis conducted in support of the research studies that involve use of stale nuclides as tracers. 4. To serve a training function and provide potential new investigators with an opportunity to learn about the application of stable isotope-tracer techniques in metabolic/nutritional research.

质谱技术，特别是那些需要测量生物基质中稳定（重）核素富集的技术，已成为研究各种病理生理条件下营养和代谢的重要工具。 CNRU内的营养/代谢研究计划在过去几年中特别推动了这一领域的发展，并继续致力于更好地定义和表征伴随各种病理生理状态的营养物质，燃料和底物代谢的变化。 一名兼职经理将与一名训练有素的全职技术人员一起支持该核心设施。质谱分析设备的主要功能有四个：1。提供体液、组织和呼气中示踪剂及其衍生物的稳定同位素富集的及时和准确测定。 2. 协助开发血液组织和尿液中化合物同位素分析的新方法，以满足参与中心资助研究计划的研究者的需求。 3. 为支持涉及使用陈旧核素作为示踪剂的研究而进行的所有分析提供质量控制。 4. 提供培训功能，并为潜在的新研究人员提供机会，了解稳定同位素示踪技术在代谢/营养研究中的应用。