PCR-based Aquatic Microbe Detection in Health Education

基于PCR的水生微生物检测在健康教育中的应用

• 批准号: 6690896
• 负责人: Karen Graf
• 金额: $ 10万
• 依托单位: EDVOTEK, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6690896
• 关键词: aquatic organism; biofilm; biotechnology; educational resource design /development; experimental designs; health education; molecular biology; polymerase chain reaction; public health; science education; water microbiology; water quality; water supply

DESCRIPTION (provided by applicant): Unsafe drinking water results in 9 million deaths annually, occurring primarily in developing countries lacking adequate sanitation. Water contamination is a significant public health concern in rural areas, especially in regions of heavy agriculture. Corroding pipelines allow biofilm formation and the subsequent contamination of urban drinking water. Health education is essential for increasing public awareness of this issue. In this proposal, Edvotek(R) will investigate the feasibility of inquiry-based health educational resources for microbial detection in water. A market survey of educators indicated that water classroom activities do not include DNA-based experiments. The proposed educational products will build on molecular biology concepts and equipment currently available in high schools. The polymerase chain reaction (PCR) facilitates microbial detection in a rapid, cost-effective manner. In Phase I we will research an educational PCR-based system for the detection of Escherichia Coli, associated with contaminated water. We will also develop a microbial DNA water extraction system. The educational resources developed in this proposal will complement existing classroom experiments. Additionally, we propose strategies for connecting these products to the National Science Education Standards. The impacts of the proposed project are 1) a sensitivity to public health issues; and 2) an appreciation of biotechnology in everyday living.

不安全的饮用水每年导致900万人死亡，主要发生在缺乏适当卫生设施的发展中国家。水污染是农村地区，特别是重农业地区的一个重大公共卫生问题。腐蚀的管道允许生物膜的形成和随后的城市饮用水污染。健康教育对于提高公众对这一问题的认识至关重要。在这项提案中，Edvotek（R）将调查基于调查的健康教育资源用于水中微生物检测的可行性。对教育工作者的市场调查表明，水课堂活动不包括基于DNA的实验。拟议的教育产品将建立在分子生物学的概念和设备目前在高中。聚合酶链反应（PCR）有助于以快速、成本效益高的方式进行微生物检测。在第一阶段，我们将研究一个教育PCR为基础的系统，用于检测大肠杆菌，与污染的水。我们还将开发微生物DNA水提取系统。本提案中开发的教育资源将补充现有的课堂实验。此外，我们还提出了将这些产品与国家科学教育标准相联系的策略。拟议项目的影响是：1）对公共卫生问题的敏感性; 2）对日常生活中生物技术的欣赏。