Educational Strategies to Prevent Abuse of Household Chemicals

防止滥用家用化学品的教育策略

• 批准号: 7748118
• 负责人: Karen Graf
• 金额: $ 15.18万
• 依托单位: EDVOTEK, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7748118
• 关键词: Address; Aerosols; Apoptosis; Biological Assay; Biological Sciences; Biology; Carbon Dioxide; Cardiovascular system; Cell Culture Techniques; Cell Death; Cell Survival; Cell division; Cells; Chemicals; Child; Cultured Cells; Drug abuse; Educational process of instructing; Exercise; Gases; Growth; Hand; Health; Hearing; Household; Humidity; Incubated; Incubators; Individual; Inhalant dose form; Insecta; Laboratories; Learning; Link; Liquid substance; Liver; Lung; Measurement; Measures; Mind; Modeling; Necrosis; Nervous system structure; Nitrites; Pharmaceutical Preparations; Phase; Plastics; Publishing; Relative (related person); Reproducibility; Research; Research Design; School-Age Population; Science; Small Business Innovation Research Grant; Solvents; Students; System; Technology; Teenagers; Temperature; Toxic effect; Toxicology; United States; abstracting; base; cell determination; cell growth; design; experience; forgetting; high school; inhalation drug abuse; middle school; planetary Atmosphere; prevent; public health relevance; research study; science education; tissue culture; tool

DESCRIPTION (provided by applicant): Inhalants, which include aerosols, volatile liquids, gases, and nitrites, have found increasing use as mind-altering drugs especially among middle school- and high school-aged children. Inhalants have devastating effects on the nervous system, as well as the lungs, liver, and circulatory system. This SBIR proposal addresses the need to teach about the effects of inhalants in high school biology using Sf9 insect cell culture, a system that lends itself to high schools in terms of its affordability, reproducibility, and relative ease of use. Insect cell will be incubated in the presence of inhalants and cells toxicity determined by cell growth and survival, as well as measurements of apoptosis vs. necrosis as the form of cell death. We will also develop assessment tools for educational strategies to prevent abuse of inhalants that are linked to the National Science Education Standards. PUBLIC HEALTH RELEVANCE: Most high schools have not utilized insect cell culture for laboratory teaching activities. While cell culture studies are copiously published, little has been done to fulfill the needs for high school biology, technology, or multi-disciplinary sciences. We propose to make these available in a format that also teaches the dangers of misuse of household items as drugs.

描述(申请人提供)：吸入剂，包括气雾剂、挥发性液体、气体和亚硝酸盐，已被越来越多地用作改变精神的药物，特别是在初中生和高中生中。吸入剂对神经系统以及肺、肝和循环系统都有毁灭性的影响。这项SBIR建议解决了使用Sf9昆虫细胞培养在高中生物学中教授吸入剂影响的必要性，Sf9昆虫细胞培养系统在可负担性、可重复性和相对易用性方面适合高中。昆虫细胞将在吸入剂和细胞毒性的存在下孵育，细胞毒性由细胞生长和存活以及作为细胞死亡形式的凋亡和坏死的测量确定。我们还将开发教育战略的评估工具，以防止与国家科学教育标准挂钩的吸入剂滥用。与公共卫生相关：大多数高中还没有将昆虫细胞培养用于实验室教学活动。虽然细胞培养的研究被大量发表，但在满足高中生物学、技术学或多学科科学的需求方面做得很少。我们建议以一种形式提供这些信息，同时也教导人们将家用物品误用为毒品的危险。