XRAY SCATTERING ON MG2+ PROMOTED STRUCT CHANGE OF ENDO & EXONUCLEASE FEN 1

这个42 kDa的核酸内切/核酸外切酶(襟翼核酸内切酶和 5‘-核酸外切酶)FEN-1在DNA复制和损伤中起重要作用 修理。我们进行了初步的溶液散射实验 野生型fen-1和a-mg2的存在和缺失 修复功能失活的定点突变体。虽然 高通量同步加速器使我们能够研究这些蛋白质 相当低的浓度(低至1毫克/毫升)，我们的初步结果 说明蛋白质需要在一个相当低的水平上测量 温度(约5 0摄氏度)而不是15 0摄氏度 这次进行了初步测量。进一步的研究将是 通过提交单独的实验方案来执行。平行 中子散射研究正在进行中。

This 42 kDa endo-/exonuclease (flap endonuclease and 5'-exonuclease) FEN-1 is important in DNA replication and damage repair. We conducted preliminary solution scattering experiments in the presence and absence of Mg2+ on the wild type FEN-1 and a site-directed mutant whose repair function is inactivated. Although the high-flux synchrotron beam allowed us to study these proteins at fairly low concentrations (as low as 1 mg/ml), our preliminary results suggest that the proteins needs to be measured at a rather low temperature (around 5 0C) instead of 15 0C where most of the preliminary measurements were done this time. Further studies will be performed by submitting a separate experimental proposal. Parallel neutron scattering studies are in progress.