Memory following CaMKII Loss in Hippocampal Subregions

描述（由申请人提供）：这些研究旨在确定α - camkii在记忆形成中的作用。具体来说，α - camkii缺失对不同海马亚区（CA1，齿状回）的影响将被检查。α - camkii的缺失将通过当代基因敲除技术和靶向病毒载体输注来完成。电生理分析将确定α - camkii缺失对长期增强（LTP）的影响，LTP是一种学习和记忆的细胞模型。行为学分析将检验α - camkii缺失对海马各亚区介导的特定记忆功能的影响。这些研究的最终目标是进一步了解人类记忆形成的分子机制。由疾病（如阿尔茨海默氏症、科尔萨科夫氏症）、衰老或损伤引起的海马功能障碍导致严重的记忆丧失。了解海马体依赖性记忆形成的分子机制，有助于深入了解由该脑区功能障碍引起的疾病。

DESCRIPTION (provided by applicant): These studies are designed to determine the role of alpha-CaMKII in memory formation. Specifically, the effects of alpha-CaMKII deletion in distinct hippocampus subregions (CA1, dentate gyrus) will be examined. Deletion of alpha-CAMKII will be accomplished using contemporary knockout technology and targeted viral-vector infusions. Electrophysiological analyses will determine the effects of alpha-CaMKII deletion on long-term potentiation (LTP), a cellular model of learning and memory. Behavioral analyses will examine the effects of alpha-CaMKII loss on specific mnemonic functions mediated by each hippocampus subregion. The ultimate goal of these studies is to further our understanding of the molecular mechanisms that underlie memory formation in humans. Hippocampal dysfunction caused by disease (e.g., Alzheimer's, Korsakoff's), aging or damage produces severe memory loss. Understanding the molecular mechanisms that underlie hippocampus-dependent memory formation should provide insight into disorders caused by the dysfunction of this brain region.