Memory following CaMKII Loss in Hippocampal Subregions

海马分区 CaMKII 丢失后的记忆

• 批准号: 7007255
• 负责人: Brian J Wiltgen
• 金额: $ 4.88万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7007255
• 关键词: behavior test; biological models; calmodulin dependent protein kinase; conditioning; dentate gyrus; electrophysiology; enzyme activity; gene deletion mutation; gene targeting; hippocampus; learning; long term potentiation; memory; molecular biology; molecular psychobiology; neural plasticity; neurochemistry; postdoctoral investigator; protein structure function; psychobiology; tissue /cell culture; transfection /expression vector

DESCRIPTION (provided by applicant): These studies are designed to determine the role of alpha-CaMKII in memory formation. Specifically, the effects of alpha-CaMKII deletion in distinct hippocampus subregions (CA1, dentate gyrus) will be examined. Deletion of alpha-CAMKII will be accomplished using contemporary knockout technology and targeted viral-vector infusions. Electrophysiological analyses will determine the effects of alpha-CaMKII deletion on long-term potentiation (LTP), a cellular model of learning and memory. Behavioral analyses will examine the effects of alpha-CaMKII loss on specific mnemonic functions mediated by each hippocampus subregion. The ultimate goal of these studies is to further our understanding of the molecular mechanisms that underlie memory formation in humans. Hippocampal dysfunction caused by disease (e.g., Alzheimer's, Korsakoff's), aging or damage produces severe memory loss. Understanding the molecular mechanisms that underlie hippocampus-dependent memory formation should provide insight into disorders caused by the dysfunction of this brain region.

描述（由申请人提供）：这些研究旨在确定α-CaMKII在记忆形成中的作用。具体而言，将检查不同海马亚区（CA 1，齿状回）中α-CaMK II缺失的影响。将使用当代敲除技术和靶向病毒载体输注来完成α-CAMKII的缺失。电生理学分析将确定α-CaMKII缺失对长时程增强（LTP）（学习和记忆的细胞模型）的影响。行为分析将检查α-CaMKII损失对每个海马子区域介导的特定记忆功能的影响。这些研究的最终目标是进一步了解人类记忆形成的分子机制。由疾病引起的海马功能障碍（例如，老年痴呆症、科萨科夫症）、衰老或损伤会导致严重的记忆丧失。了解依赖于大脑皮层的记忆形成的分子机制，将有助于我们深入了解这一大脑区域功能障碍所导致的疾病。

项目成果

The hippocampus plays a selective role in the retrieval of detailed contextual memories.

• DOI: 10.1016/j.cub.2010.06.068
• 发表时间: 2010-08-10
• 期刊: CURRENT BIOLOGY
• 影响因子: 9.2
• 作者: Wiltgen, Brian J.;Zhou, Miou;Cai, Ying;Balaji, J.;Karlsson, Mikael Guzman;Parivash, Sherveen N.;Li, Weidong;Silvaz, Alcino J.
• 通讯作者: Silvaz, Alcino J.