IMAGING VIRUS PARTICLES W/ 300 KEV ELECTRON CRYOMICROSCOPE

使用 300 KEV 电子低温显微镜对病毒颗粒进行成像

• 批准号: 6611296
• 负责人: Wah Chiu
• 金额: $ 13.47万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6611296
• 关键词: biomedical resource; infection; technology /technique; virus

This project is aimed at evaluating the potential of a 300 keV electron cryomicroscope with a field emission gun and a liquid helium cryostage. Such an instrument is only available in Japan. We froze samples of P22 phage on EM grids in Houston and shipped them under liquid nitrogen to Dr. Y. Fujiyoshi s laboratory in Japan. Joanita Jakana and Wah Chiu performed the imaging in Japan. A few sets of quality images were obtained in a week of intensive work. Our subsequent computer analysis indicated that the damping of the computed transform of the images taken in Japan was not as steep as that of the 400 keV images taken with equivalent defocus in Houston. This preliminary data has provided the results needed to develop a grant proposal submitted to NSF for acquisition of such an instrument, which costs ~US$2 million, in the US. We have obtained a strong matching fund commitment from Baylor s administration to support this grant application. Notice of funding of $ 1 million from NSF has just been received today.

该项目旨在评估300 keV的潜力 带场致发射枪和液氦的电子低温显微镜 冷冻台 这种仪器只有在日本才有。 我们冻结 在休斯顿的EM网格上的P22噬菌体样品，并将它们运送到 液氮给Y博士Fujiyoshi在日本的实验室。 若安妮塔 Jakana和Wah Chiu在日本进行了成像。 几套 在一周的紧张工作中获得了高质量的图像。 我们 随后的计算机分析表明， 在日本拍摄的图像的计算变换并不像 在休斯敦以等效散焦拍摄的400 keV图像的能量。 这些初步数据提供了开发一个 向NSF提交了购买此类仪器的赠款提案， 在美国的售价约为200万美元。 我们得到了一个强大的 贝勒政府为支持这一目标而承诺的配套资金 补助金申请 来自NSF的100万美元资金的通知刚刚 今天收到了。