Control of cell fate decisions in neurogenesis: use of embryonic stem cells to investigate key signalling systems and gene expression programs

神经发生中细胞命运决定的控制：利用胚胎干细胞研究关键信号系统和基因表达程序

• 批准号: DP140103743
• 负责人: Prof Colin Pouton
• 金额: $ 27.49万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP140103743
• 关键词: Control; cell; fate; decisions; neurogenesis

Human embryonic stem cells (hESC) have the potential to provide an unlimited source of specific subtypes of human neurons for basic studies in neuroscience and biomedical applications. The use of hESC is limited at present by a lack of control over lineage commitment during differentiation in vitro. This project will use engineered reporter hESC lines to investigate which cell signalling pathways and gene expression programs are involved in controlling cell fate. The project will result in improved protocols for hESC differentiation allowing enrichment of cultures with specific neuronal subtypes, and significant advances in the understanding of neuronal lineage commitment and maturation during brain development.

人类胚胎干细胞（hESC）有潜力为神经科学和生物医学应用的基础研究提供特定亚型的人类神经元的无限来源。hESC的使用目前由于在体外分化过程中缺乏对谱系承诺的控制而受到限制。该项目将使用工程报告细胞hESC细胞系来研究哪些细胞信号通路和基因表达程序参与控制细胞命运。该项目将改进hESC分化的方案，从而丰富特定神经元亚型的培养，并在理解大脑发育过程中神经元谱系的承诺和成熟方面取得重大进展。