MAD PHASING OF SELENOMETHIONYL CA2+ FREE MANNOSE BINDING PROTEIN C
MAD PHASING OF SELENOMETHIONYL CA2+ FREE MANNOSE BINDING PROTEIN C
批准号:
6586517
负责人:
William I Weis
金额:
$14.32万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
已结题
起止时间:
2002-03-01 至 2003-02-28
中文摘要
C型凝集素是一类依赖于钙离子的碳水化合物结合蛋白
发生可逆构象变化的蛋白质,
它们不能结合Ca 2+,因此碳水化合物,在温和的酸性pH值。
为了理解这种结构转变,我们已经结晶了
甘露糖结合蛋白C的凝集素结构域的无钙形式。
我们已经用硒代甲硫氨酸代替了
蛋氨酸,我们建议解决结构的多波长
硒甲硫酰蛋白的异常分散定相,使用
可调1-5AD光束线收集数据。
英文摘要
C-type lectins are a family of Ca2+-dependent carbohydrate-binding
proteins that undergo a reversible conformational change that renders
them unable to bind Ca2+, hence carbohydrates, at mildly acid pH. In
order to understand this structural transition, we have crystallized
the Ca2+-free form of the lectin domain of mannose-binding protein C.
We have prepared this protein with selenomethionine substituted for
methionine, and we propose to solve the structure by multiwavelength
anomalous dispersion phasing of the selenomethionyl protein, using the
tuneable 1-5AD beamline to collect the data.
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