DEVELOPMENT OF CELL POLARITY IN BUDDING YEAST

芽殖酵母细胞极性的发展

• 批准号: 6691682
• 负责人: DAVID G DRUBIN
• 金额: $ 33.49万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6691682
• 关键词: Saccharomyces cerevisiae; actins; biological polymorphism; biological signal transduction; cell cycle proteins; cell growth regulation; cellular polarity; cytoskeletal proteins; endocytosis; fungal genetics; gene mutation; guanine nucleotide binding protein; guanosinetriphosphatases; laboratory rabbit; microorganism growth; microorganism reproduction; molecular genetics; protein kinase; protein protein interaction; protein structure function; yeast two hybrid system

DESCRIPTION (Verbatim from the applicant's abstract): S. cerevisiae is an excellent organism for studies of cell polarity development due to its powerful genetics, its simplicity, the ability to observe polarity development synchronously in a cell population, and existence of numerous mutations and molecular markers for cell polarity. Studies will determine how Cdc42 GTPase controls actin organization, and how actin mediates polarized endocytosis. Thirty-seven site-directed cdc42 mutants will be characterized biochemically in permeabilized yeast and in actin-assembly competent yeast extracts to relate biochemical activities to in vivo functions, and used in genetic screens to identify components of downstream pathways. Roles for Cdc42 effector Pak kinases in morphogenesis will be elucidated by genetic and chemical-genetic strategies. Special cdc42 alleles defective in pseudohyphal growth will be studied to provide a deeper understanding of the molecular basis for fungal dimorphism, a characteristic of many fungal pathogens. Finally, how a complex set of interacting proteins links endocytic membranes to the Arp2/3 complex, and how these proteins are regulated by novel Ark kinases discovered in this laboratory, will be investigated.

描述（摘自申请人摘要）：S. cerevisiae是一种