CORE--TRANSGENIC MOUSE

引入被操纵的基因并删除或修改内源基因的能力 动物的生殖系是生物学上的一项重大技术进步，使研究人员能够回答 必须在整个动物模型系统中分析的基因功能问题。 转基因和基因敲除动物在深入了解发育基因调控机制、免疫系统内的细胞相互作用以及癌基因对生长和分化的影响方面发挥了重要作用。自上次审查以来，对这些服务的需求大幅增加，大约每两年翻一番。2000年初招聘了一名新的设施经理，他在培育基因敲除小鼠方面具有特定的专业知识。在1999日历年，有21项服务请求。2003年，完成了84项请求(注射了76个DNA构建物，注射了8个ES细胞系)。同行审查资助用户的数量已从上次审查时的9个增加到2003年历年的15个。来自这15名同行评审资助的研究人员的使用代表了六个不同的研究项目和福克斯·蔡斯癌症中心(Fccc)的所有三个部门。99%(99%)的设施使用是由同行审查资助的调查人员完成的。正如上一次申请中所描述的，我们计划增加所需的冷冻保存服务。冷冻保存已经建立，并已成为一项常规服务。它目前由11名调查人员使用。到目前为止，共有88行文字被保存下来。这项服务的提供带来了几个明显的好处，包括能够将小鼠品系存入银行以供未来研究，缓解现有小鼠群体空间的压力，提供繁殖繁殖不能繁殖的小鼠品系的手段，并提供保险，防止在小鼠群体发生灾难性事件时失去不可替代的小鼠品系。先前的审查指出，需要提供冷冻保存和基因敲除服务，以及增加设施的使用。所有这三点都得到了充分的解决。冷冻保存和基因打靶现在都是例行公事，成功率为100%。自1998年以来，生产率和使用率分别提高了200%和100%。

The ability to introduce manipulated genes and delete or modify endogenous genes in the germline of animals is a major technological advance in biology, enabling investigators to answer questions about gene function which must be analyzed in a whole animal model system. Transgenic and knockout animals have been instrumental in providing insights into mechanisms of developmental gene regulation, cellular interactions within the immune system, and the effect of oncogenes on growth and differentiation. Demand for these services has grown significantly since the last review, approximately doubling every two years. A new Facility Manager was recruited in early 2000 with a specific expertise in generating knockout mice. In calendar year 1999, there were 21 requests for services. In 2003, 84 requests were completed (76 DNA Constructs Injected, 8 ES Cell Lines Injected). The number of peer-reviewed funded users has increased from nine at the last review to 15 in calendar year 2003. The use from these 15 peer-reviewed funded investigators represents six different Research Programs and all three Divisions of the Fox Chase Cancer Center (FCCC). Ninety-nine percent (99%) of Facility usage is by peer-reviewed funded investigators. As described in the last application, we planned to add needed cryopreservation services. Cryopreservation has been established and has become a routine service. It is currently used by 11 investigators. A total of 88 lines have been preserved to date. The availability of this service has resulted in several clear benefits, including the ability to bank mouse lines for future study, to relieve pressure on existing mouse colony space, to provide a means for propagating reproductively incompetent mouse lines, and to provide insurance against the loss of irreplaceable mouse lines should a catastrophic event occur in the mouse colony. The previous review noted a need to provide cryopreservation and gene knockout services as well as to increase Facility usage. All three points have been fully addressed. Both cryopreservation and gene targeting are now accomplished routinely, with a 100% success rate. Productivity and usage have increased by 200% and 100%, respectively, since 1998.