In Vivo Imaging of Leukocyte-Endothelial Dynamics
白细胞内皮动力学的体内成像
基本信息
- 批准号:6874846
- 负责人:
- 金额:$ 15.1万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2003
- 资助国家:美国
- 起止时间:2003-04-15 至 2007-03-31
- 项目状态:已结题
- 来源:
- 关键词:Sjogren&aposs syndromecapillary bedcell cell interactioncell migrationclinical researchconfocal scanning microscopyconjunctivaconjunctivitisdrug adverse effecteye disordereyelid disordergraft versus host diseasehuman subjecthypersensitivityindomethacinintravital microscopyiridocyclitiskeratoconjunctivitis siccaleukocyte adhesion moleculesleukocytesnonsteroidal antiinflammatory agentpathologic processprednisolonescleravascular endotheliumvision tests
项目摘要
DESCRIPTION (provided by applicant): The process of leukocyte migration across the vascular endothelial barrier is fundamental to the process of inflammation and the response to infection. We will quantitate the effect of various medications such as topical corticosteroids, oral nonsteroidal anti-inflammatory drugs, and mast cell stabilizers in several of these processes. We have applied intravital microscopy in animal systems to visualize, quantitate and analyze this process. Recent advances in confocal microscopy have allowed a European group to quantitate leukocyte endothelial rolling and sticking in the microvasculature of the human eye. Combining our clinical expertise in confocal microscopy and our experience analyzing leukocyte vascular interactions, we propose to utilize in vivo confocal technology to quantitate leukocyte rolling and arrest in 4 different human vascular beds: the limbus, conjuctiva, episclera, and sclera. With these three specific aims: Aim one, we propose to image rolling and sticking of leukocytes in four different normal ocular vascular beds: the conjunctiva, limbus, episclera, and sclera. Aim two, we will compare leukocyte-endothelial dynamics in specific vascular beds in seven disease states: a) allergic seasonal conjunctivitis, b) Sjogren's syndrome and dry eye, c) blepharitis, d) graft versus host disease (GVHD), e) episcleritis, f) scleritis, and g) anterior uveitis. Aim three, we will determine the effect of medications including topical prednisolone acetate, a mast cell stabilizer (optipranolol), or an oral nonsteroidal anti-inflammatory drug (indomethacin) on endothelial-leukocyte dynamics in diseases for which each is frequently prescribed. Our studies will directly clarify the pathogenesis of several troublesome and rarely studied ocular disease processes. These studies will elucidate the mechanism by which medications alter these processes. Most importantly these studies will quantitate a fundamental human biological process in microvascular beds that have not previously been imaged.
描述(由申请方提供):白细胞迁移穿过血管内皮屏障的过程是炎症过程和感染反应的基础。我们将定量各种药物如局部皮质类固醇、口服非甾体抗炎药和肥大细胞稳定剂在其中几个过程中的作用。我们已经在动物系统中应用活体显微镜来可视化,定量和分析这一过程。 最近在共聚焦显微镜的进展,使一个欧洲小组定量白细胞内皮滚动和粘附在微血管的人的眼睛。 结合我们在共聚焦显微镜和我们的经验分析白细胞血管相互作用的临床专业知识,我们建议利用在体内共聚焦技术来定量白细胞滚动和逮捕4个不同的人类血管床:利姆布斯,结膜,巩膜外层,巩膜。 有这三个具体的目标:目标一,我们提出的图像滚动和粘白细胞在四个不同的正常眼血管床:结膜,利姆布斯,巩膜外层,巩膜。 目的二,我们将比较七种疾病状态下特定血管床中的白细胞内皮动力学:a)过敏性季节性结膜炎,B)干燥综合征和干眼,c)睑缘炎,d)移植物抗宿主病(GVHD),e)巩膜外层炎,f)巩膜炎,和g)前葡萄膜炎。 目标三,我们将确定药物(包括外用醋酸泼尼松龙、肥大细胞稳定剂(奥替洛尔)或口服非甾体类抗炎药(消炎痛))对疾病中内皮白细胞动力学的影响,每种药物都是经常开处方的。 我们的研究将直接阐明几个棘手的和很少研究的眼病过程的发病机制。 这些研究将阐明药物改变这些过程的机制。 最重要的是,这些研究将量化以前没有成像的微血管床中的基本人类生物学过程。
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Histopathological features of conjunctival sarcoid nodules using noninvasive in vivo confocal microscopy.
使用无创体内共聚焦显微镜观察结膜肉样结节的组织病理学特征。
- DOI:10.1001/archopht.123.2.274
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Wertheim,MichaelS;Mathers,WilliamD;Suhler,EricB;Wilson,DavidJ;Rosenbaum,JamesT
- 通讯作者:Rosenbaum,JamesT
In vivo confocal microscopy of keratic precipitates.
角质沉淀的体内共聚焦显微镜。
- DOI:10.1001/archopht.122.12.1773
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Wertheim,MichaelS;Mathers,WilliamD;Planck,StephenJ;Martin,TammyM;Suhler,EricB;Smith,JustineR;Rosenbaum,JamesT
- 通讯作者:Rosenbaum,JamesT
Non-invasive detection of multinucleated giant cells in the conjunctiva of patients with sarcoidosis by in-vivo confocal microscopy.
体内共聚焦显微镜无创检测结节病患者结膜中的多核巨细胞。
- DOI:10.1080/09273940600826471
- 发表时间:2006
- 期刊:
- 影响因子:3.3
- 作者:Wertheim,MichaelS;Mathers,WilliamD;Lim,Lyndell;Watkins,AngelaS;Mackensen,Friederike;O'Malley,JeanP;Rosenbaum,JamesT
- 通讯作者:Rosenbaum,JamesT
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WILLIAM D MATHERS其他文献
WILLIAM D MATHERS的其他文献
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{{ truncateString('WILLIAM D MATHERS', 18)}}的其他基金
In Vivo Imaging of Leukocyte-Endothelial Dynamics
白细胞内皮动力学的体内成像
- 批准号:
6614375 - 财政年份:2003
- 资助金额:
$ 15.1万 - 项目类别:
In Vivo Imaging of Leukocyte-Endothelial Dynamics
白细胞内皮动力学的体内成像
- 批准号:
6732733 - 财政年份:2003
- 资助金额:
$ 15.1万 - 项目类别:
MEIBOMIAN GLAND DYSFUNCTION IN CHRONIC BLEPHARITIS
慢性睑缘炎的睑板腺功能障碍
- 批准号:
2163864 - 财政年份:1992
- 资助金额:
$ 15.1万 - 项目类别:
MEIBOMIAN GLAND DYSFUNCTION IN CHRONIC BLEPHARITIS
慢性睑缘炎的睑板腺功能障碍
- 批准号:
3267372 - 财政年份:1992
- 资助金额:
$ 15.1万 - 项目类别:
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