A Genetic Investigation of Regeneration in Drosophila

果蝇再生的遗传研究

• 批准号: 7111815
• 负责人: Rachel Smith-Bolton
• 金额: $ 4.32万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7111815
• 关键词: Drosophilidae; angiogenesis; arthropod genetics; cell cycle; cell proliferation; clinical research; gene induction /repression; genetic regulation; genetic screening; human genetic material tag; human tissue; imaginal disc; molecular cloning; postdoctoral investigator; regeneration

DESCRIPTION (provided by applicant): A fascinating biological phenomenon in which cells grow and divide despite a context and history that should prevent proliferation is the regeneration of damaged tissues. Various organisms and tissues are capable of regenerating, and some tissues such as frog limbs and fruit fly larvae lose their ability to regenerate over time. Therefore, these organisms contain genes that can overcome a cell's normal program and induce cell division, and genes that can prevent this cell cycle progression. The work proposed here is designed to identify genes that regulate regenerative growth. First, an assay system will be developed through which regeneration in Drosophila may be studied genetically. Second, genetic screens will be conducted to isolate mutations that affect regenerative growth after tissue ablation. Third, the genes and proteins that control this process will be identified and characterized. Genes that regulate regenerative growth may also regulate the progression of cancers. The identification of genes that specifically regulate cell division during regeneration and cancer progression may provide unique therapeutic targets.

描述(申请人提供)：一种令人着迷的生物学现象，在这种现象中，细胞生长和分裂，而不管背景和历史，应该防止增殖是受损组织的再生。各种生物和组织能够再生，一些组织，如青蛙的四肢和果蝇幼虫，随着时间的推移失去了再生的能力。因此，这些生物含有可以克服细胞正常程序并诱导细胞分裂的基因，以及可以阻止细胞周期进展的基因。这里提出的工作是为了识别控制再生生长的基因。首先，将开发一种检测系统，通过该系统可以从遗传学角度研究果蝇的再生。其次，将进行基因筛查，以分离影响组织切除后再生生长的突变。第三，将识别和表征控制这一过程的基因和蛋白质。调控再生生长的基因也可能调控癌症的进展。识别在再生和癌症进展过程中特定调控细胞分裂的基因可能提供独特的治疗靶点。

项目成果

Regenerative growth in Drosophila imaginal discs is regulated by Wingless and Myc.

• DOI: 10.1016/j.devcel.2009.04.015
• 发表时间: 2009-06
• 期刊: DEVELOPMENTAL CELL
• 影响因子: 11.8
• 作者: Smith-Bolton, Rachel K.;Worley, Melanie I.;Kanda, Hiroshi;Hariharan, Iswar K.
• 通讯作者: Hariharan, Iswar K.