MASS SPECTROMETRY CORE

质谱核心（核心A）的使命是提供具有成本效益的、最先进的 为药理学和药物研究中心的研究人员提供仪器和分析专业知识 毒理学。MS Core有10个质谱仪可供一般使用，包括5个三重质谱仪。 四极杆LC/MS仪器和两个GC/MS仪器。预算中要求的资金达到50% 费用分摊购买新一代三重四极杆LC/MS仪器，以取代其中一个 淘汰现有仪器，以进一步增强目标分析物的低水平定量。五个项目将 参与本研究中心，核心将由他们每个人使用。核心的主要用途 将用于鉴定和表征生理液体中的小分子代谢物， 通过同位素稀释质谱法定量类二十烷酸、洋地黄素和氧化脂质。核心 人员积极参与该中心的研究目标，并将承担短期项目，如 提高色谱技术和仪器灵敏度以及探索衍生化 化学以增强目标化合物的电离性质。核心将由Dr. 生物化学教授Richard Caprioli与大卫L. Hachey教授 药理学和生物化学，以及核心的日常职责将由四个全职实验室完成 人事和行政助理。实验室人员协助用户开发分析 方法，设计实验，开发标准操作程序，保持质量控制 记录仪器性能和维护历史，为研究者进行常规测定， 培训学生和研究员在理论和实践方面的MS.核心有一个定期的培训 该计划每年提供六次，为新用户提供仪器操作方面的专业培训， 样品制备。此外，中心的研究人员将有机会获得更先进的 MS研究中心的仪器，这些仪器将通过 根据需要与卡普里奥利医生达成协议因此，核心满足日常分析需求， 中心调查员，并确保新的分析能力将可满足更苛刻的要求， 超出常规服务核心范围的研究挑战。

The mission of the Mass Spectrometry Core (Core A) is to provide cost-effective, state-of-the-art instrumentation and analytical expertise to investigators in the Research Center for Pharmacology and Drug Toxicology. The MS Core has ten mass spectrometers available for general use, including five triple quadrupole LC/MS instruments and two GC/MS instruments. Funds are requested in the budget to 50% cost-share the purchase of a new generation triple quadrupole LC/MS instrument to replace one of the obsolete current instruments to further enhance low level quantitation of target analytes. Five projects will participate in this Research Center and the Core will be used by each of them. The primary use of the Core will be for identification and characterization of small molecule metabolites in physiologic fluids and quantitation of eicosanoids, prostaglandins and oxidized lipids by isotope dilution mass spectrometry. Core personnel actively participate in the research goals of this center and will undertake short projects, such as those to enhance chromatographic techniques and instrument sensitivity and to explore derivatization chemistry to enhance the ionization properties of target compounds. The Core will be managed by Dr. Richard Caprioli, Professor of Biochemistry in conjunction with Dr. David L. Hachey, Professor of Pharmacology and Biochemistry, and routine duties of the core will be done by four full-time laboratory personnel and an administrative assistant. Laboratory personnel assist users in developing analytical methods, designing experiments, developing standard operating procedures, maintaining quality control records of instrument performance and maintenance history, performing routine assays for investigators and training students and fellows in the theoretical and practical aspects of MS. The core has a regular training program given six times per year in which it offers new users specialized training in instrument operation and sample preparation. Furthermore, the center investigators will have access to more advanced instrumentation in the MS Research Center, and these will be made available to investigators through arrangements with Dr. Caprioli as needs dictate. Thus, the Core satisfies the routine analytical needs of center investigators and assures that new analytical capabilities will be available to meet more demanding research challenges that fall beyond the scope of a routine service core.