3D super-resolved measurement of molecular dynamics in the cell membrane

细胞膜分子动力学的 3D 超分辨测量

• 批准号: 2745057
• 金额: --
• 依托单位: University of Strathclyde
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2745057
• 关键词: 3D; super; resolved; measurement; molecular

This project will explore a new method of obtaining 3D Fluorescence correlation Spectroscopy (FCS) measurements utilising a new STED-FCS-FLIM microscope, which, when compared against typical methods, will allow for much quicker measurements whilst retaining high spatial resolution.Increasing the rate at which these measurements are obtained is essential as the current, slower, methods are not quick enough to capture and quantify many of the essential molecular processes and dynamics which occur within the cell membrane in the required detail. One such process which occurs within the cell membrane is insulin-regulated membrane trafficking, which is known to be impaired with diseases such as Type 2 diabetes, where insulin transfer is somehow inhibited. This project aims to increase our understanding of such processes within fat cells, seeking to therefore increase our understanding of the mechanisms behind diseases such as Type 2 diabetes, through an increased understanding of molecular dynamics within cell membranes, and the development of new methods for 3D super-resolved measurements.

该项目将探索一种利用新型STED-FCS-FLIM显微镜获得3D荧光相关光谱（FCS）测量的新方法，与典型方法相比，该方法将允许更快的测量，同时保持高空间分辨率。提高获得这些测量的速率至关重要，因为当前，较慢，这些方法不够快，不能以所需的细节捕获和定量细胞膜内发生的许多基本分子过程和动力学。发生在细胞膜内的一个这样的过程是胰岛素调节的膜运输，已知其在2型糖尿病等疾病中受损，其中胰岛素转移以某种方式被抑制。该项目旨在增加我们对脂肪细胞内这些过程的理解，从而通过增加对细胞膜内分子动力学的理解，以及开发3D超分辨测量的新方法，来增加我们对2型糖尿病等疾病背后机制的理解。